Pfeuffer T, Gaugler B, Metzger H
FEBS Lett. 1983 Nov 28;164(1):154-60. doi: 10.1016/0014-5793(83)80040-4.
Homologous and heterologous complexes between catalytic and GTP-binding components can be isolated by means of immobilized succinyldeacetylforskolin (forskolin-Sepharose). A heterologous complex is formed by reconstitution of forskolin-Sepharose bound catalytic function from rabbit myocardial membranes with the homogenous [3H]methyl-GTP-binding protein from duck erythrocyte membranes. Analysis of the reconstituted complex by sodium dodecyl sulfate polyacrylamide gelelectrophoresis reveals that only the Mr 42 000 component of the GTP-binding protein's Mr 42 000/Mr 35 000 heterodimer contributes to the formation of active adenylate cyclase.
催化成分与GTP结合成分之间的同源和异源复合物可通过固定化琥珀酰去乙酰佛司可林（佛司可林-琼脂糖）分离。异源复合物是通过将来自兔心肌膜的与佛司可林-琼脂糖结合的催化功能与来自鸭红细胞膜的均一[3H]甲基-GTP结合蛋白重组而形成的。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳对重组复合物进行分析表明，GTP结合蛋白的Mr 42 000/Mr 35 000异二聚体中只有Mr 42 000成分有助于活性腺苷酸环化酶的形成。
