小鼠3T3B和3T3B/SV40细胞中延伸因子1α的甲基化
Methylation of elongation factor 1 alpha in mouse 3T3B and 3T3B/SV40 cells.
作者信息
Coppard N J, Clark B F, Cramer F
出版信息
FEBS Lett. 1983 Dec 12;164(2):330-4. doi: 10.1016/0014-5793(83)80311-1.
PMID:6317455
Abstract
Two-dimensional gel electrophoretic (NEPHGE) analysis of proteins from mouse 3T3B and 3T3B/SV40 cells labelled with [methyl-3H]methionine in the presence of cycloheximide have revealed that the elongation factor 1 alpha (EF-1 alpha) in these cells is methylated and that the extent of methylation is higher in the SV40 transformed cell type. It is suggested that methylation may account for differences in growth properties for the different cell types.
摘要
在放线菌酮存在的情况下，用[甲基-³H]甲硫氨酸标记的小鼠3T3B和3T3B/SV40细胞的蛋白质二维凝胶电泳（非平衡pH梯度凝胶电泳，NEPHGE）分析显示，这些细胞中的延伸因子1α（EF-1α）发生了甲基化，并且在SV40转化细胞类型中甲基化程度更高。有人提出，甲基化可能是不同细胞类型生长特性差异的原因。
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