Carmichael A J, Mossoba M M, Riesz P
FEBS Lett. 1983 Dec 12;164(2):401-5. doi: 10.1016/0014-5793(83)80325-1.
The hydroxyl and superoxide anion spin adducts of DMPO and 4-MePyBN, respectively, were obtained during photoirradiation of adriamycin and daunomycin solutions with visible light. Ethanol and dimethyl sulfoxide did not scavenge hydroxyl radicals in the photoirradiated drug solutions. Furthermore, the hydroxyl-DMPO spin adduct is not formed in the photolysis of air-free drug solutions, indicating that hydroxyl radicals are not directly produced in the photochemical reactions. Instead, the observed hydroxyl-DMPO is formed from the decay of the superoxide anion-DMPO spin adduct. The mechanism for generating the superoxide anion radical appears to be a direct electron transfer from the photoexcited adriamycin and daunomycin to dissolved oxygen.
分别在可见光照射阿霉素和柔红霉素溶液的过程中，得到了DMPO的羟基自旋加合物和4-MePyBN的超氧阴离子自旋加合物。乙醇和二甲基亚砜不会清除光照射药物溶液中的羟基自由基。此外，在无氧药物溶液的光解过程中不会形成羟基-DMPO自旋加合物，这表明在光化学反应中不会直接产生羟基自由基。相反，观察到的羟基-DMPO是由超氧阴离子-DMPO自旋加合物的衰变形成的。产生超氧阴离子自由基的机制似乎是光激发的阿霉素和柔红霉素将电子直接转移给溶解氧。
