Green M R, Basketter D A, Couchman J R, Rees D A
Dev Biol. 1983 Dec;100(2):506-12. doi: 10.1016/0012-1606(83)90243-9.
Epidermal growth factor (EGF), a low-molecular-weight polypeptide (G. Carpenter and S. Cohen, 1979, Annu. Rev. Biochem. 48, 193-216), stimulates the proliferation and keratinisation of cultured embryonic epidermis (S. Cohen, 1965, Dev. Biol. 12, 394-407) and promotes epidermal growth, thickening, and keratinisation when injected into neonatal mice (S. Cohen and G.A. Elliott, 1963, J. Invest. Dermatol, 40, 1-5). We have determined the distribution of the available receptors for epidermal growth factor in rat skin using autoradiography following incubation of explants with 125I-labelled mouse EGF. EGF receptors are detected on the epithelial cells overlying the basement membranes of the epidermis, sebaceous gland, and regions of the hair follicle all of which have proliferative capacity. In marked contrast, tissues which have started to differentiate and lost their growth potential, carry either an undetectable or sharply reduced number of EGF receptors. The EGF receptor number and receptor affinity of epidermal basal cells freshly isolated from rats of increasing age has also been determined. We find that receptor affinity remains unchanged (3.3 nM) but that basal cell surface receptor number decreases markedly with age. This decrease in receptor number is similar in trend to the known drop in basal cell [3H]thymidine labelling index which occurs over the same time period. The data suggest that the distribution of EGF receptors and EGF cell surface receptor number in skin are important in the spatial and temporal control of epithelial proliferation.
表皮生长因子（EGF）是一种低分子量多肽（G. 卡彭特和S. 科恩，1979年，《生物化学年度评论》48卷，193 - 216页），它能刺激培养的胚胎表皮的增殖和角质化（S. 科恩，1965年，《发育生物学》12卷，394 - 407页），并且当注射到新生小鼠体内时能促进表皮生长、增厚和角质化（S. 科恩和G.A. 埃利奥特，1963年，《皮肤病学研究杂志》40卷，1 - 5页）。我们用125I标记的小鼠EGF孵育外植体后，通过放射自显影法测定了大鼠皮肤中表皮生长因子的可用受体的分布。在表皮、皮脂腺和毛囊具有增殖能力的区域的基底膜上方的上皮细胞上检测到了EGF受体。与之形成鲜明对比的是，已经开始分化并失去生长潜能的组织，其EGF受体数量要么检测不到，要么急剧减少。我们还测定了从不同年龄大鼠新鲜分离的表皮基底细胞的EGF受体数量和受体亲和力。我们发现受体亲和力保持不变（3.3 nM），但基底细胞表面受体数量随年龄显著减少。受体数量的这种减少趋势与同一时期已知的基底细胞[3H]胸腺嘧啶核苷标记指数的下降相似。数据表明，皮肤中EGF受体的分布和EGF细胞表面受体数量在上皮增殖的空间和时间控制中很重要。
