骨髓来源的巨噬细胞作为3型小鼠肝炎病毒的复制靶标。
Bone-marrow derived macrophages as targets for the replication of mouse hepatitis virus type 3.
作者信息
Schindler L, Klimetzek V, Kirchner H
出版信息
Immunol Lett. 1983;7(2):107-10. doi: 10.1016/0165-2478(83)90042-1.
Bone-marrow (BM) derived macrophages are sensitive target cells for replication of mouse hepatitis virus type 3 (MHV3). These cells can be grown in large numbers and the percentage of defined macrophages increased until day 10 when 100% of the cells represented macrophages. MHV3 replicated within these cells to high titers and caused the formation of multi-nucleated giant cells. This effect was seen with very low virus inocula in BM macrophages of C57BL/6 mice that are highly susceptible to in vivo infection with MHV3 whereas macrophages from resistant A/J mice did not show a cytopathic effect at these virus doses. 1000-fold higher virus doses, however, caused the cytopathic effect in macrophages of both C57BL/6 and A/J mice.
骨髓（BM）来源的巨噬细胞是3型小鼠肝炎病毒（MHV3）复制的敏感靶细胞。这些细胞可以大量培养，特定巨噬细胞的百分比会持续增加，直到第10天，此时100%的细胞均为巨噬细胞。MHV3在这些细胞内大量复制，并导致多核巨细胞的形成。在对体内感染MHV3高度敏感的C57BL/6小鼠的骨髓巨噬细胞中，极低剂量的病毒接种就可观察到这种效应，而在相同病毒剂量下，抗性A/J小鼠的巨噬细胞未表现出细胞病变效应。然而，高1000倍的病毒剂量可在C57BL/6和A/J小鼠的巨噬细胞中引发细胞病变效应。
Zotero 插件