Mózsik G, Morón F, Fiegler M, Jávor T, Nagy L, Patty I, Tárnok F
Int J Tissue React. 1983;5(3):263-78.
The effects were studied of different doses of prostacyclin (PG12), atropine and cimetidine on the gastric secretory responses of four-hour pylorus-ligated rats and on the gastric mucosal damage produced by intragastric administration of 0.6 M HCl, to determine the cytoprotective doses of PG12, atropine and cimetidine. In another series of observations, gastric mucosal damage was produced by intragastric administration of 0.6 M HCl and the effects were studied of cytoprotective doses of PG12, atropine and cimetidine on the number and severity of gastric lesions, in connection with the biochemical changes of the rat gastric fundic mucosa. The drugs were given intraperitoneally 30 min before the application of the necrotizing agent, and the animals were killed one hour later. During the experiments, the tissue levels of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and lactate were measured enzymatically, and the tissue content of cyclic adenosine monophosphate (cAMP) was determined by radioimmunoassay. The specimens were taken from the gastric fundic mucosa of groups of animals treated with saline solution (absolute control), with 0.6 M HCl (pathological control) and with 0.6 M HCl plus PG12, atropine and cimetidine (given in cytoprotective doses). The ratio of ATP.ADP-1, adenylate pool (ATP + ADP + AMP) and energy charge (ATP + 0.5 ADP.ATP + ADP + AMP-1) were calculated in all groups of animals. It was found that: (i) PG12 (in a dose of 5 micrograms.kg-1) atropine (in a dose of 0.025 mg.kg-1) and cimetidine (in a dose of 2.5 micrograms.kg-1) have cytoprotective effects; (ii) the gastric fundic mucosal damage produced by intragastric administration of 0.6 M HCl appears as a result of a positive metabolic adaptation of the gastric fundic mucosa; (iii) the development of gastric cytoprotection by PG12, atropine and cimetidine give rise to very different changes in the tissue levels of ATP, ADP, AMP and cAMP.(ABSTRACT TRUNCATED AT 400 WORDS)
研究了不同剂量的前列环素（PG12）、阿托品和西咪替丁对幽门结扎4小时大鼠胃分泌反应以及对胃内给予0.6 M盐酸所致胃黏膜损伤的影响，以确定PG12、阿托品和西咪替丁的细胞保护剂量。在另一系列观察中，胃内给予0.6 M盐酸造成胃黏膜损伤，并研究了PG12、阿托品和西咪替丁的细胞保护剂量对胃损伤数量和严重程度的影响，以及与大鼠胃底黏膜生化变化的关系。在应用坏死剂前30分钟腹腔注射药物，1小时后处死动物。实验期间，通过酶法测定三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）和乳酸的组织水平，并通过放射免疫分析法测定环磷酸腺苷（cAMP）的组织含量。标本取自用生理盐水（绝对对照）、0.6 M盐酸（病理对照）以及0.6 M盐酸加PG12、阿托品和西咪替丁（给予细胞保护剂量）处理的动物组的胃底黏膜。计算了所有动物组的ATP/ADP-1、腺苷酸库（ATP + ADP + AMP）和能荷（ATP + 0.5 ADP/ATP + ADP + AMP-1）。结果发现：（i）PG12（剂量为5微克/千克）、阿托品（剂量为0.025毫克/千克）和西咪替丁（剂量为2.5微克/千克）具有细胞保护作用；（ii）胃内给予0.6 M盐酸所致胃底黏膜损伤是胃底黏膜正性代谢适应的结果；（iii）PG12、阿托品和西咪替丁产生的胃细胞保护作用在ATP、ADP、AMP和cAMP的组织水平上引起非常不同的变化。（摘要截断于400字）
