Nakazawa H, Nakano M M, Ogawara H
J Antibiot (Tokyo). 1983 Nov;36(11):1543-8. doi: 10.7164/antibiotics.36.1543.
Introduction of hybrid plasmids, which were constructed by ligation of pCR1 or pMN1 vector plasmid and SalI restriction endonuclease cleaved segments of Streptomyces cacaoi chromosome, resulted in the production of new beta-lactamase and penicillin-binding protein in Escherichia coli. The beta-lactamase and penicillin-binding protein were not from S. cacaoi but rather induced by the plasmids. Close relationship was observed between plasmids and penicillin-binding proteins but not with beta-lactamase.
通过将pCR1或pMN1载体质粒与可可链霉菌染色体经SalI限制性内切酶切割的片段连接构建的杂种质粒导入大肠杆菌后，产生了新的β-内酰胺酶和青霉素结合蛋白。β-内酰胺酶和青霉素结合蛋白并非来自可可链霉菌，而是由质粒诱导产生的。观察到质粒与青霉素结合蛋白之间存在密切关系，但与β-内酰胺酶无关。
