Fulchignoni-Lataud M C, Roux J M
J Cell Physiol. 1984 Jan;118(1):34-8. doi: 10.1002/jcp.1041180108.
In a family of clonal lines derived from the Reuber H 35 rat hepatoma, four electrophoretically distinct molecular forms of uridine kinase (UK I, II, III, and IV) have been characterized. They are the same as those found in foetal rat liver. Different UK profiles occur in these cell lines, and no strict correlation could be established between the state of differentiation of the cells and the form of UK expressed. A clone of somatic hybrid cells between line p4 (form 1 only) and Fu5-5 (forms II, III, and IV) that does not express form I indicates that p4 cells may lack a factor controlling the polymerization of form I. This variety of clonal cell lines was used to study the uptake and phosphorylation of labeled uridine. The results suggest a relationship between the UK form present and the rate uridine phosphorylation by the intact cells.
在源自鲁伯H 35大鼠肝癌的一组克隆系中，已鉴定出尿苷激酶的四种电泳性质不同的分子形式（UK I、II、III和IV）。它们与在胎鼠肝脏中发现的形式相同。这些细胞系中出现了不同的尿苷激酶谱，并且在细胞的分化状态与所表达的尿苷激酶形式之间无法建立严格的相关性。p4细胞系（仅形式I）和Fu5-5细胞系（形式II、III和IV）之间的体细胞杂交细胞克隆不表达形式I，这表明p4细胞可能缺乏控制形式I聚合的因子。利用这种多样的克隆细胞系研究了标记尿苷的摄取和磷酸化。结果表明完整细胞中存在的尿苷激酶形式与尿苷磷酸化速率之间存在关联。
