猿猴病毒40 a蛋白与复制起点处有缺失的DNA的结合。
Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.
作者信息
Lewton B A, DeLucia A L, Tegtmeyer P
出版信息
J Virol. 1984 Jan;49(1):9-13. doi: 10.1128/JVI.49.1.9-13.1984.
Abstract
Previous studies with wild-type simian virus 40 DNA have shown that the sequence 5'-GAGGC-3' directs the binding of A protein (T antigen). The functional origin of replication contains four recognition pentanucleotides each of which is separated by a single base pair and arranged a two pairs of direct repetitions that are inverted relative to each other. Analysis of A protein binding to a series of nonviable mutants progressively deleting these contact sites leads to the following conclusions: (i) stable binding of subunits of A protein to three origin pentanucleotides is not sufficient for the initiation of DNA replication, (ii) the stability of DNA binding depends on interactions between bound protein subunits, and (iii) a single pentanucleotide is sufficient to bind and orient a subunit of A protein.
摘要
先前对野生型猿猴病毒40 DNA的研究表明，序列5'-GAGGC-3'指导A蛋白（T抗原）的结合。功能性复制起点包含四个识别五核苷酸，每个五核苷酸由单个碱基对隔开，并排列成两对彼此反向的直接重复序列。对与一系列逐步删除这些接触位点的无活力突变体的A蛋白结合进行分析，得出以下结论：（i）A蛋白亚基与三个起点五核苷酸的稳定结合不足以启动DNA复制；（ii）DNA结合的稳定性取决于结合的蛋白质亚基之间的相互作用；（iii）单个五核苷酸足以结合并定向A蛋白的一个亚基。
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