猿猴病毒40 DNA复制起始位点内的关键空间需求。
Critical spatial requirement within the origin of simian virus 40 DNA replication.
作者信息
Cohen G L, Wright P J, DeLucia A L, Lewton B A, Anderson M E, Tegtmeyer P
出版信息
J Virol. 1984 Jul;51(1):91-6. doi: 10.1128/JVI.51.1.91-96.1984.
Abstract
We inserted a single base pair into the center of a 27-base-pair palindrome within the replication origin of simian virus 40. The mutation did not directly alter the symmetry of the palindrome or the protein-binding sequences within the palindrome. DNA binding studies showed that subunits of the simian virus 40 A protein (T antigen) bound to each of the four recognition pentanucleotides in the origin palindrome but did so with reduced affinity in comparison with wild-type origins. The mutant origin cloned in a plasmid DNA failed to replicate in COS cells. Thus, precise spatial interactions among subunits of A protein are necessary for stable origin binding and are crucial for subsequent steps in the initiation of DNA replication. Furthermore, any possible functional interactions of the simian virus 40 A protein with cellular DNA would require a great fidelity of protein binding arrangements to initiate cellular DNA replication.
摘要
我们在猴病毒40复制起点内一个27个碱基对的回文序列中心插入了一个单碱基对。该突变并未直接改变回文序列的对称性或回文序列内的蛋白质结合序列。DNA结合研究表明，猴病毒40 A蛋白（T抗原）的亚基与起点回文序列中的四个识别五核苷酸中的每一个结合，但与野生型起点相比，其结合亲和力降低。克隆到质粒DNA中的突变起点在COS细胞中无法复制。因此，A蛋白亚基之间精确的空间相互作用对于稳定的起点结合是必要的，并且对于DNA复制起始的后续步骤至关重要。此外，猴病毒40 A蛋白与细胞DNA之间任何可能的功能相互作用都需要蛋白质结合排列具有高度保真度才能启动细胞DNA复制。
相似文献
1
Critical spatial requirement within the origin of simian virus 40 DNA replication.
J Virol. 1984 Jul;51(1):91-6. doi: 10.1128/JVI.51.1.91-96.1984.
2
The T-antigen-binding domain of the simian virus 40 core origin of replication.
J Virol. 1987 Jul;61(7):2143-9. doi: 10.1128/JVI.61.7.2143-2149.1987.
3
Topography of simian virus 40 A protein-DNA complexes: arrangement of protein bound to the origin of replication.
J Virol. 1983 Apr;46(1):151-61. doi: 10.1128/JVI.46.1.151-161.1983.
4
Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.
J Virol. 1984 Jan;49(1):9-13. doi: 10.1128/JVI.49.1.9-13.1984.
5
Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication.
J Virol. 1983 Apr;46(1):143-50. doi: 10.1128/JVI.46.1.143-150.1983.
6
Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication.
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6491-5. doi: 10.1073/pnas.77.11.6491.
7
Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
J Virol. 1990 Feb;64(2):509-18. doi: 10.1128/JVI.64.2.509-518.1990.
8
Protein-DNA interactions at the simian virus 40 origin of replication.
Biochim Biophys Acta. 1988 Dec 20;951(2-3):388-95. doi: 10.1016/0167-4781(88)90111-x.
9
E1 recognition sequences in the bovine papillomavirus type 1 origin of DNA replication: interaction between half sites of the inverted repeats.
J Virol. 1995 Jun;69(6):3789-98. doi: 10.1128/JVI.69.6.3789-3798.1995.
10
Sequence-specific functions of the early palindrome domain within the SV40 core origin of replication.
Nucleic Acids Res. 1989 Nov 25;17(22):9279-89.
引用本文的文献
1
ATM and ATR activities maintain replication fork integrity during SV40 chromatin replication.
PLoS Pathog. 2013;9(4):e1003283. doi: 10.1371/journal.ppat.1003283. Epub 2013 Apr 4.
2
Preformed hexamers of SV40 T antigen are active in RNA and origin-DNA unwinding.
Nucleic Acids Res. 2002 Jul 15;30(14):3192-201. doi: 10.1093/nar/gkf416.
3
The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.
Mol Cell Biol. 2001 Feb;21(4):1098-110. doi: 10.1128/MCB.21.4.1098-1110.2001.
4
Replication but not transcription of simian virus 40 DNA is dependent on nuclear domain 10.
J Virol. 2000 Oct;74(20):9694-700. doi: 10.1128/jvi.74.20.9694-9700.2000.
5
The simian virus 40 core origin contains two separate sequence modules that support T-antigen double-hexamer assembly.
J Virol. 2000 Sep;74(18):8589-600. doi: 10.1128/jvi.74.18.8589-8600.2000.
6
Two regions of simian virus 40 T antigen determine cooperativity of double-hexamer assembly on the viral origin of DNA replication and promote hexamer interactions during bidirectional origin DNA unwinding.
J Virol. 1999 Mar;73(3):2201-11. doi: 10.1128/JVI.73.3.2201-2211.1999.
7
Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
J Virol. 1997 May;71(5):3972-85. doi: 10.1128/JVI.71.5.3972-3985.1997.
8
Mutation of the cyclin-dependent kinase phosphorylation site in simian virus 40 (SV40) large T antigen specifically blocks SV40 origin DNA unwinding.
J Virol. 1993 Aug;67(8):4992-5002. doi: 10.1128/JVI.67.8.4992-5002.1993.
9
Species-specific functional interactions of DNA polymerase alpha-primase with simian virus 40 (SV40) T antigen require SV40 origin DNA.
Mol Cell Biol. 1994 May;14(5):3176-85. doi: 10.1128/mcb.14.5.3176-3185.1994.
10
Sequences flanking the pentanucleotide T-antigen binding sites in the polyomavirus core origin help determine selectivity of DNA replication.
J Virol. 1995 Dec;69(12):7570-8. doi: 10.1128/JVI.69.12.7570-7578.1995.
本文引用的文献
1
Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.
J Virol. 1984 Jan;49(1):9-13. doi: 10.1128/JVI.49.1.9-13.1984.
2
Nonviable mutants of simian virus 40 with deletions near the 3' end of gene A define a function for large T antigen required after onset of viral DNA replication.
J Virol. 1983 Sep;47(3):487-94. doi: 10.1128/JVI.47.3.487-494.1983.
3
Binding of simian virus 40 large T antigen from virus-infected monkey cells to wild-type and mutant viral replication origins.
J Mol Biol. 1983 Aug 25;168(4):791-808. doi: 10.1016/s0022-2836(83)80075-8.
4
SV40 deletion mutants lacking the 21-bp repeated sequences are viable, but have noncomplementable deficiencies.
Nucleic Acids Res. 1983 Mar 11;11(5):1601-16. doi: 10.1093/nar/11.5.1601.
5
High efficiency polyoma DNA transfection of chloroquine treated cells.
Nucleic Acids Res. 1983 Mar 11;11(5):1295-308. doi: 10.1093/nar/11.5.1295.
6
Topography of simian virus 40 A protein-DNA complexes: arrangement of protein bound to the origin of replication.
J Virol. 1983 Apr;46(1):151-61. doi: 10.1128/JVI.46.1.151-161.1983.
7
Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication.
J Virol. 1983 Apr;46(1):143-50. doi: 10.1128/JVI.46.1.143-150.1983.
8
Deletion mapping of DNA regions required for SV40 early region promoter function in vivo.
J Mol Appl Genet. 1982;1(5):457-81.
9
DNA binding properties of simian virus 40 temperature-sensitive A proteins.
J Virol. 1982 Nov;44(2):458-66. doi: 10.1128/JVI.44.2.458-466.1982.
10
Regulatory mutants of simian virus 40. Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes.
J Mol Biol. 1982 Apr 15;156(3):531-48. doi: 10.1016/0022-2836(82)90265-0.
Zotero 插件