恶性转化对培养的小鼠乳腺细胞中细胞视黄醇结合蛋白的影响。

Effect of malignant transformation upon the cellular retinoid binding proteins in cultured murine mammary cells.

作者信息

Bunk M J, Telang N T, Sarkar N H

出版信息

Cancer Lett. 1983 Aug;20(1):83-92. doi: 10.1016/0304-3835(83)90191-x.

Abstract

Cellular retinol (CRBP) and retinoic acid binding proteins (CRABP) were measured in normal (C57Bl) and Murine Mammary Tumor Virus (MuMTV)-induced murine mammary tumor cells (C57BlfRIII) grown in monolayer culture. High speed supernatant fractions (cytosols) from transformed cells contained elevated levels of CRBP and CRABP (0.276 and 1.410 pmol/mg protein, respectively) compared to cytosol from non-transformed murine mammary cells which contained only low levels of CRBP (0.099 pmol/mg protein). Our findings suggest that malignant transformation of mammary epithelial cells, induced by MuMTV infection, results in changes in the expression of cellular retinoid binding proteins and possibly in the sensitivity of these cells to various retinoids.

摘要

在单层培养中生长的正常（C57Bl）小鼠和鼠乳腺肿瘤病毒（MuMTV）诱导的小鼠乳腺肿瘤细胞（C57BlfRIII）中检测了细胞视黄醇（CRBP）和视黄酸结合蛋白（CRABP）。与仅含有低水平CRBP（0.099 pmol/mg蛋白质）的未转化小鼠乳腺细胞的胞质溶胶相比，来自转化细胞的高速上清液部分（胞质溶胶）含有升高水平的CRBP和CRABP（分别为0.276和1.410 pmol/mg蛋白质）。我们的研究结果表明，MuMTV感染诱导的乳腺上皮细胞恶性转化导致细胞类视黄醇结合蛋白表达的变化，并可能导致这些细胞对各种类视黄醇的敏感性发生变化。