Hazelrigg T, Levis R, Rubin G M
Cell. 1984 Feb;36(2):469-81. doi: 10.1016/0092-8674(84)90240-x.
P-element-mediated DNA transformation was used to generate transformants carrying segments of DNA from the white locus of D. melanogaster. The vast majority of transduced copies of an 11.7 or a 14.3 kb segment of DNA from white successfully rescued the white- eye-color phenotype when inserted in many different chromosomal locations. However, two transformants with abnormal eye pigmentation--apparently a consequence of the genomic positions of the transduced white gene--were also recovered. In all seven cases tested, autosomal insertions of white, which is dosage-compensated in its normal location on the X chromosome, retained the property of dosage compensation. In contrast to the relative insensitivity of eye-color pigmentation and dosage compensation to genomic position, the transduced white DNA segments differed widely in their interactions with the zeste mutation, ranging from greater than normal repression by zeste to insensitivity to the presence of zeste.
利用P因子介导的DNA转化来产生携带来自黑腹果蝇白眼基因座DNA片段的转化体。当插入许多不同的染色体位置时，来自白眼基因的11.7 kb或14.3 kb DNA片段的绝大多数转导拷贝成功挽救了白眼颜色表型。然而，也获得了两个眼色素沉着异常的转化体——显然这是转导的白眼基因的基因组位置导致的结果。在所有测试的7个案例中，白眼基因在常染色体上的插入（白眼基因在其正常的X染色体位置是剂量补偿的）保留了剂量补偿特性。与眼色色素沉着和剂量补偿对基因组位置的相对不敏感相反，转导的白眼DNA片段与zeste突变的相互作用差异很大，从zeste对其的抑制作用大于正常情况到对zeste存在不敏感。
