Cohen D, Nir I
Exp Eye Res. 1983 Dec;37(6):575-82. doi: 10.1016/0014-4835(83)90133-1.
Anionic sites on the surface of cultured pigment epithelium cells from normal and dystrophic rats were studied. Cytochemical procedures and quantitative analysis were employed in order to determine distribution patterns and labeling densities. Anionic sites, in general, were localized with cationized ferritin at pH 7.0. The presence and density of sialic acid residues on the pigment epithelium cell surface were evaluated with colloidal iron at pH 1.8. Dystrophic cells contained 20% more cationized ferritin-binding sites as compared with normal pigment epithelium cells. With colloidal iron, an 18% increase in labeling density was measured in dystrophic pigment epithelium cells. Pretreatment with neuraminidase reduced subsequent labeling with colloidal iron by 93-97%. Thus, the presence of sialic acid residues on the surface of pigment epithelium cells and a significant increase in its content on the surface of dystrophic cells were established.
对来自正常和营养不良大鼠的培养色素上皮细胞表面的阴离子位点进行了研究。采用细胞化学方法和定量分析来确定分布模式和标记密度。一般来说，阴离子位点在pH 7.0时用阳离子铁蛋白定位。在pH 1.8时用胶体铁评估色素上皮细胞表面唾液酸残基的存在和密度。与正常色素上皮细胞相比，营养不良细胞含有多20%的阳离子铁蛋白结合位点。用胶体铁测量，营养不良色素上皮细胞的标记密度增加了18%。用神经氨酸酶预处理可使随后用胶体铁的标记减少93 - 97%。因此，确定了色素上皮细胞表面存在唾液酸残基，且其在营养不良细胞表面的含量显著增加。
