Wong K H, Kisilevsky R
Exp Mol Pathol. 1984 Feb;40(1):122-34. doi: 10.1016/0014-4800(84)90070-4.
Four hours after the administration of ethionine and in the face of an 80-90% depletion of hepatic ATP there is a twofold increase in cAMP. This increase in cAMP is shown not to be effected by a reduction in phosphodiesterase activity. Both the low Km and high Km forms of the enzyme retain their activity. The purification of liver plasma membranes is not affected by ethionine. There is a significant 20% increase in plasma membrane adenylate cyclase activity following acute ethionine intoxication. This was demonstrable using either ATP or 5'-adenylyl-imidodiphosphate as substrates. The plasma membranes, isolated in the presence of Ca2+, have a 20-fold higher basal adenylate cyclase activity then previously reported and are not further stimulated by GTP or NaF. A modified protocol for isolating cAMP from the adenylate cyclase reaction is described.
给予乙硫氨酸四小时后，在肝脏ATP耗竭80 - 90%的情况下，环磷酸腺苷（cAMP）增加了两倍。结果表明，cAMP的这种增加并非由磷酸二酯酶活性降低所致。该酶的低Km和高Km形式均保持其活性。乙硫氨酸不影响肝细胞膜的纯化。急性乙硫氨酸中毒后，细胞膜腺苷酸环化酶活性显著增加20%。以ATP或5'-腺苷酰亚胺二磷酸为底物时均可证实这一点。在Ca2+存在下分离得到的细胞膜，其基础腺苷酸环化酶活性比先前报道的高20倍，且不受GTP或NaF的进一步刺激。本文描述了一种从腺苷酸环化酶反应中分离cAMP的改良方法。
