Buckland P R, Rees Smith B
FEBS Lett. 1984 Jan 23;166(1):109-14. doi: 10.1016/0014-5793(84)80054-x.
TSH receptors from guinea pig thyroid and epididymal fat have been covalently crosslinked to 125I-labelled TSH conjugated to N-hydroxysuccinimidyl-4-azidobenzoate. Analysis by SDS-PAGE and autoradiography showed bands corresponding to TSH subunits (Mr 14 000) and intact TSH (Mr 28 000; subunits crosslinked) and two at higher Mr separated by 14 000. The latter bands represented one or two subunits of TSH crosslinked to a subunit of the TSH receptor with Mr 57 000 (fat) or 60 000 (thyroid). These Mr values were reduced by trypsin treatment to 43 000 and 50 000, respectively. Analysis under nonreducing conditions showed that both fat and thyroid receptors have a second disulphide linked subunit of Mr 30 000.
豚鼠甲状腺和附睾脂肪中的促甲状腺激素（TSH）受体已与共价交联至与N - 羟基琥珀酰亚胺 - 4 - 叠氮苯甲酸共轭的125I标记的TSH上。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）和放射自显影分析显示，有对应于TSH亚基（分子量14000）和完整TSH（分子量28000；亚基交联）的条带，以及两条分子量较高且相差14000的条带。后两条带代表与分子量为57000（脂肪）或60000（甲状腺）的TSH受体亚基交联的一个或两个TSH亚基。经胰蛋白酶处理后，这些分子量值分别降至43000和50000。在非还原条件下分析表明，脂肪和甲状腺受体都有一个分子量为30000的通过二硫键连接的第二个亚基。
