Morgan J P, Chesebro J H, Pluth J R, Puga F J, Schaff H V
J Am Coll Cardiol. 1984 Feb;3(2 Pt 1):410-8. doi: 10.1016/s0735-1097(84)80028-5.
The calcium transients associated with contraction in human working myocardium were recorded by use of the bioluminescent protein, aequorin, a substance that emits light when it combines with calcium ion (Ca++). Small amounts of aequorin were microinjected into superficial cells of human atrial and ventricular muscle obtained from tissue routinely excised and discarded at the time of cardiac surgery. Light output, an index of intracellular Ca++, and isometric tension development were recorded at 37.5 degrees C at 1 to 5 second intervals of stimulation. Light increases much more quickly than tension and decreases toward basal levels by the time that peak tension is reached. The configuration and time course of the aequorin signal in human myocardium and its responses to inotropic interventions are similar to those recorded in lower mammalian species. The calcium transient appears to be dominated by the release and uptake of Ca++ from intracellular stores under all conditions studied. These results indicate that aequorin is a useful tool for studying the effects of drugs and disease states on cardiac excitation-contraction coupling in human beings as well as in lower animals.
利用生物发光蛋白水母发光蛋白记录了人类工作心肌收缩时的钙瞬变。水母发光蛋白是一种与钙离子（Ca++）结合时会发光的物质。在心脏手术时常规切除并丢弃的组织中获取少量水母发光蛋白，将其微量注射到人类心房和心室肌的表层细胞中。在37.5摄氏度下，以1至5秒的刺激间隔记录光输出（细胞内Ca++的指标）和等长张力的发展。光的增加比张力快得多，在达到峰值张力时，光朝着基础水平下降。人类心肌中水母发光蛋白信号的形态和时程及其对变力干预的反应与在低等哺乳动物中记录到的相似。在所研究的所有条件下，钙瞬变似乎都由细胞内储存库中Ca++的释放和摄取所主导。这些结果表明，水母发光蛋白是研究药物和疾病状态对人类以及低等动物心脏兴奋 - 收缩偶联影响的有用工具。
