通过电子显微镜确定大小的轮状病毒RNA片段。
Rotavirus RNA segments sized by electron microscopy.
作者信息
Rixon F, Taylor P, Desselberger U
出版信息
J Gen Virol. 1984 Jan;65 ( Pt 1):233-9. doi: 10.1099/0022-1317-65-1-233.
PMID:6319571
Abstract
The RNA segments of bovine rotavirus (UK Compton strain) were measured by electron microscopy in mixtures containing phi X174 double-stranded DNA as an internal reference molecule. The numbers of base pairs per segment were calculated and compared with data obtained by other procedures (comparative RNA gel electrophoresis; co-electrophoresis of cDNAs in denaturing gels; sequencing).
摘要
在含有φX174双链DNA作为内部参照分子的混合物中，通过电子显微镜测量了牛轮状病毒（英国康普顿毒株）的RNA片段。计算了每个片段的碱基对数，并与通过其他方法（比较RNA凝胶电泳；cDNA在变性凝胶中的共电泳；测序）获得的数据进行了比较。
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