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展神经副核运动神经元的超微结构和电生理特性:猫的细胞内辣根过氧化物酶研究

Ultrastructural and electrophysiological properties of accessory abducens nucleus motoneurones: an intracellular horseradish peroxidase study in the cat.

作者信息

Destombes J, Durand J, Gogan P, Gueritaud J P, Horcholle-Bossavit G, Tyc-Dumont S

出版信息

Neuroscience. 1983 Dec;10(4):1317-32. doi: 10.1016/0306-4522(83)90114-8.

DOI:10.1016/0306-4522(83)90114-8
PMID:6320049
Abstract

The physiological and morphological (light and electron microscopy) properties of six retractor bulbi motoneurones were analysed using the technique of intracellular recording and intracellular labelling with horseradish peroxidase. The retractor bulbi motoneurones were identified by antidromic invasion and orthodromic responses following stimulation of trigeminal afferents were studied. Two of these motoneurones were examined ultrastructurally. Terminal boutons forming synapses with labelled soma, labelled proximal and distal dendrites were characterized. Serial sections allowed the axon hillock to be analyzed and the initial segment of a presumed motoneurone to be observed in the section where the injected motoneurone was described. The ultrastructure of unidentified elements observed in the accessory abducens nucleus is stressed.

摘要

运用细胞内记录技术以及辣根过氧化物酶细胞内标记法,分析了6个眼球退缩运动神经元的生理特性和形态学特性(光学显微镜和电子显微镜观察)。通过逆向冲动入侵来识别眼球退缩运动神经元,并研究了刺激三叉神经传入纤维后的顺向反应。对其中两个运动神经元进行了超微结构检查。对与标记的胞体、标记的近端和远端树突形成突触的终末小体进行了特征描述。连续切片使得轴丘得以分析,并且在描述注射的运动神经元的切片中观察到了一个假定运动神经元的起始段。着重强调了在外展神经副核中观察到的未识别成分的超微结构。

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