Suppr超能文献

配体结合对Nycodenz梯度中DNA浮力密度的影响。

Effect of ligand binding on the buoyant density of DNA in Nycodenz gradients.

作者信息

Ford T C, Rickwood D

出版信息

Nucleic Acids Res. 1984 Jan 25;12(2):1219-26. doi: 10.1093/nar/12.2.1219.

DOI:10.1093/nar/12.2.1219
PMID:6320119
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318568/
Abstract

The effect of ligand binding upon the buoyant density of DNA in Nycodenz gradients has been studied using DNAs of differing base compositions. The effect of both intercalating ligands (ethidium bromide and proflavin) and non-intercalating ligands (distamycin A, DAPI and netropsin) has been studied. The binding of intercalating ligands to DNA has essentially no effect on the buoyant density of DNA in Nycodenz gradients. The non-intercalating ligands were found to increase the buoyant density of DNA in a base specific manner. The increase in buoyant density can be interpreted in terms of disruption of the hydration shell of the DNA molecule caused by the binding of the ligand along the minor groove of the DNA helix.

摘要

利用不同碱基组成的DNA，研究了配体结合对Nycodenz梯度中DNA浮力密度的影响。研究了嵌入配体（溴化乙锭和原黄素）和非嵌入配体（偏端霉素A、DAPI和纺锤菌素）的作用。嵌入配体与DNA的结合对Nycodenz梯度中DNA的浮力密度基本没有影响。发现非嵌入配体以碱基特异性方式增加DNA的浮力密度。浮力密度的增加可以解释为配体沿DNA螺旋小沟结合导致DNA分子水化层破坏。

相似文献

1
Effect of ligand binding on the buoyant density of DNA in Nycodenz gradients.配体结合对Nycodenz梯度中DNA浮力密度的影响。
Nucleic Acids Res. 1984 Jan 25;12(2):1219-26. doi: 10.1093/nar/12.2.1219.
2
Buoyant densities of macromolecules, macromolecular complexes, and cell organelles in Nycodenz gradients.在 Nycodenz 梯度中大分子、大分子复合物和细胞器的浮力密度。
Anal Biochem. 1983 Jan;128(1):232-9. doi: 10.1016/0003-2697(83)90370-6.
3
Nycodenz: a new nonionic iodinated gradient medium.尼可登茨：一种新型非离子型碘化梯度介质。
Anal Biochem. 1982 Jun;123(1):23-31. doi: 10.1016/0003-2697(82)90618-2.
4
Isopycnic centrifugation of mammalian metaphase chromosomes in Nycodenz.在 Nycodenz 中对哺乳动物中期染色体进行等密度离心。
Exp Cell Res. 1984 May;152(1):204-11. doi: 10.1016/0014-4827(84)90245-3.
5
The effect of silver ion binding and pH on the buoyant density of DNA and its use in fractionating heterogeneous DNA.银离子结合和pH对DNA浮力密度的影响及其在分离异质DNA中的应用。
Biochim Biophys Acta. 1976 Apr 2;425(4):451-62. doi: 10.1016/0005-2787(76)90009-5.
6
Buoyant density of Escherichia coli is determined solely by the osmolarity of the culture medium.大肠杆菌的浮力密度仅由培养基的渗透压决定。
Arch Microbiol. 1995 Aug;164(2):155-7. doi: 10.1007/s002030050248.
7
Separation of endocytic vesicles in Nycodenz gradients.在 Nycodenz 梯度中分离内吞小泡。
Anal Biochem. 1984 Nov 1;142(2):455-62. doi: 10.1016/0003-2697(84)90489-5.
8
Formation of isotonic Nycodenz gradients for cell separations.用于细胞分离的等渗 Nycodenz 梯度的形成。
Anal Biochem. 1982 Aug;124(2):293-8. doi: 10.1016/0003-2697(82)90041-0.
9
The compatibility of netropsin and actinomycin binding to natural deoxyribonucleic acid.纺锤菌素与放线菌素结合于天然脱氧核糖核酸的相容性。
J Biol Chem. 1975 Apr 10;250(7):2698-702.
10
Buoyant density sedimentation of macromolecules in sodium iothalamate density gradients.大分子在碘他拉酸钠密度梯度中的浮力密度沉降
J Mol Biol. 1975 Mar 5;92(3):433-48. doi: 10.1016/0022-2836(75)90290-9.

本文引用的文献

1
X-ray diffraction and molecular model building studies of the interaction of actinomycin with nucleic acids.放线菌素与核酸相互作用的X射线衍射及分子模型构建研究。
Nature. 1963 May 11;198:538-40. doi: 10.1038/198538b0.
2
Structural considerations in the interaction of DNA and acridines.DNA与吖啶相互作用中的结构考量
J Mol Biol. 1961 Feb;3:18-30. doi: 10.1016/s0022-2836(61)80004-1.
3
Nycodenz: a new nonionic iodinated gradient medium.尼可登茨：一种新型非离子型碘化梯度介质。
Anal Biochem. 1982 Jun;123(1):23-31. doi: 10.1016/0003-2697(82)90618-2.
4
Calorimetric and spectroscopic investigation of drug-DNA interactions. I. The binding of netropsin to poly d(AT).药物与DNA相互作用的量热法和光谱法研究。I. 纺锤菌素与聚d(AT)的结合
Nucleic Acids Res. 1983 May 11;11(9):2857-70. doi: 10.1093/nar/11.9.2857.
5
Z-DNA and other non-B-DNA structures are reversed to B-DNA by interaction with netropsin.Z-DNA和其他非B-DNA结构通过与纺锤菌素相互作用而转变为B-DNA。
FEBS Lett. 1983 Apr 5;154(1):156-60. doi: 10.1016/0014-5793(83)80894-1.
6
The action of heat and ionizing radiation on the infectivity of isolated phi-X-174 DNA.热和电离辐射对分离出的φ-X-174 DNA感染性的作用。
Proc Natl Acad Sci U S A. 1968 May;60(1):251-7. doi: 10.1073/pnas.60.1.251.
7
A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.一种用于检测和分离闭环双链DNA的染料浮力密度法：HeLa细胞中的闭环DNA
Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21. doi: 10.1073/pnas.57.5.1514.
8
Echinomycin: a bifunctional intercalating antibiotic.棘霉素：一种双功能嵌入型抗生素。
Nature. 1974 Dec 20;252(5485):653-7. doi: 10.1038/252653a0.
9
[Stereochemical model of the molecular mechanism of the "recognition" of AT-pairs during the binding with DNA of the antibiotics distamycin A and netropsin].[抗生素偏端霉素A和纺锤菌素与DNA结合过程中AT碱基对“识别”分子机制的立体化学模型]
Dokl Akad Nauk SSSR. 1976 Dec 1;231(4):1006-9.
10
Interaction between synthetic analogues of quinoxaline antibiotics and nucleic acids. Changes in mechanism and specificity related to structural alterations.喹喔啉类抗生素合成类似物与核酸之间的相互作用。与结构改变相关的作用机制和特异性变化。
Biochem J. 1978 Jul 1;173(1):129-44. doi: 10.1042/bj1730129.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验