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配体结合对Nycodenz梯度中DNA浮力密度的影响。

Effect of ligand binding on the buoyant density of DNA in Nycodenz gradients.

作者信息

Ford T C, Rickwood D

出版信息

Nucleic Acids Res. 1984 Jan 25;12(2):1219-26. doi: 10.1093/nar/12.2.1219.

DOI:10.1093/nar/12.2.1219
PMID:6320119
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318568/
Abstract

The effect of ligand binding upon the buoyant density of DNA in Nycodenz gradients has been studied using DNAs of differing base compositions. The effect of both intercalating ligands (ethidium bromide and proflavin) and non-intercalating ligands (distamycin A, DAPI and netropsin) has been studied. The binding of intercalating ligands to DNA has essentially no effect on the buoyant density of DNA in Nycodenz gradients. The non-intercalating ligands were found to increase the buoyant density of DNA in a base specific manner. The increase in buoyant density can be interpreted in terms of disruption of the hydration shell of the DNA molecule caused by the binding of the ligand along the minor groove of the DNA helix.

摘要

利用不同碱基组成的DNA，研究了配体结合对Nycodenz梯度中DNA浮力密度的影响。研究了嵌入配体（溴化乙锭和原黄素）和非嵌入配体（偏端霉素A、DAPI和纺锤菌素）的作用。嵌入配体与DNA的结合对Nycodenz梯度中DNA的浮力密度基本没有影响。发现非嵌入配体以碱基特异性方式增加DNA的浮力密度。浮力密度的增加可以解释为配体沿DNA螺旋小沟结合导致DNA分子水化层破坏。

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本文引用的文献

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