成年、性未成熟的河七鳃鳗（Lampetra fluviatilis [L.] Gray）的造血神经上器官，特别涉及嗜天青白细胞。

The haematopoietic supraneural organ of adult, sexually immature river lampreys (Lampetra fluviatilis [L.] Gray) with particular reference to azurophil leucocytes.

作者信息

Kelényi G, Larsen L O

出版信息

Acta Biol Acad Sci Hung. 1976;27(1):45-56.

Abstract

The haematopoietic tissue in the supraneural organ of the freshwater river lamprey (Lampetra fluviatilis L. Gray) was studied in sexually immature animals. Besides erythro- and granulopoietic elements, macrophages, reticular cells, fibroblasts and glycogen-rich fat cells were seen. Developing granulocytes of the lamprey contain one type of azurophil granules originating from small cytoplasmic (Golgi) vesicles. The lamprey's azurophil granulocytes seem to be homologous with those of fishes. However, the granulocytes of fishes, studied thus far, show granules with only one type of inclusion, whereas in lamprey the granulocyte inclusions are variable in size and shape. Thus, lamprey granulocytes are, in this respect, reminiscent of similar cells of higher vertebrates. The PAS and alkaline phosphatase reactions, common markers of vertebrate neutrophil leucocytes, are very weak in the haematopoietic tissue granulocytes of the lamprey, and intense in the blood cells of the same animal. Lamprey granulocytes, similarly to the granulocytes of Chondrostei and Elasmobranchiata, do not stain with peroxidase, naphthol-AS-D-chloroacetate esterase and sudan black B. The haematopoietic tissue contains a relatively high number of degenerated granulocytes.

摘要

对淡水河七鳃鳗（Lampetra fluviatilis L. Gray）神经上器官中的造血组织进行了研究，研究对象为性未成熟动物。除了红细胞生成和粒细胞生成成分外，还可见巨噬细胞、网状细胞、成纤维细胞和富含糖原的脂肪细胞。七鳃鳗发育中的粒细胞含有一种源自小细胞质（高尔基体）囊泡的嗜天青颗粒。七鳃鳗的嗜天青粒细胞似乎与鱼类的嗜天青粒细胞同源。然而，迄今为止所研究的鱼类粒细胞显示颗粒仅含有一种内含物，而七鳃鳗粒细胞的内含物在大小和形状上是可变的。因此，在这方面，七鳃鳗粒细胞让人联想到高等脊椎动物的类似细胞。脊椎动物中性粒细胞的常见标记物过碘酸希夫反应（PAS）和碱性磷酸酶反应，在七鳃鳗造血组织粒细胞中非常弱，而在同一动物的血细胞中则很强。与软骨硬鳞鱼和板鳃亚纲动物的粒细胞一样，七鳃鳗粒细胞不能用过氧化物酶、萘酚-AS-D-氯乙酸酯酶和苏丹黑B染色。造血组织中含有相对大量的退化粒细胞。