Farrell R L, Ingram V M
Prog Clin Biol Res. 1983;134:263-6.
Viral transformation affects certain developmental processes by blocking the differentiation of cells such as primitive muscle cells (myoblasts) or primitive red blood cells (erythroblasts). Embryonic quail myoblasts, transformed with a temperature-sensitive (ts) mutant of Rous sarcoma virus (RSV) are blocked in differentiation at the viral permissive temperature of 35 degrees C, but fuse to form myotubes at the nonpermissive temperature of 41 degrees C. We have shown that viral transformation of quail myoblasts also causes transcription of non-muscle-specific genes, ie, globins at 35 degrees C that are not observed in uninfected myoblasts or myotubes. Transformed myoblasts shifted to 41 degrees C for 5 days still contain these globin transcripts. Both alpha-like and beta-like globins are expressed.
病毒转化通过阻断某些细胞的分化来影响特定的发育过程，这些细胞如原始肌肉细胞（成肌细胞）或原始红细胞（成红细胞）。用劳斯肉瘤病毒（RSV）的温度敏感（ts）突变体转化的胚胎鹌鹑成肌细胞，在35℃的病毒允许温度下分化受阻，但在41℃的非允许温度下融合形成肌管。我们已经表明，鹌鹑成肌细胞的病毒转化也会导致非肌肉特异性基因的转录，即在35℃时的珠蛋白，而在未感染的成肌细胞或肌管中未观察到这种情况。转移到41℃ 5天的转化成肌细胞仍然含有这些珠蛋白转录本。α样和β样珠蛋白均有表达。
