Sytkowski A J, Perrine S P, Bicknell K A, Kessler C J
Prog Clin Biol Res. 1983;134:335-43.
We have shown morphologic and biochemical evidence for erythroid differentiation in the erythropoietin-responsive cell line Rauscher murine erythroleukemia. These cells synthesize adult and presumably embryonic hemoglobin in response to erythropoietin and dimethyl sulfoxide. Clear differences are observed in some clones between different inducers. The molecular basis for these differences, which may relate to differential modes of inducer action, must be clarified. These cells possess a functional beta-adrenergic receptor/adenylyl cyclase complex remarkably similar to that found on erythrocytes of several species. The receptor density is up-regulated specifically by erythropoietin and, as such, is the first description of membrane receptor regulation by this hormone. Thus, we now have the opportunity to investigate the development of the receptor-cyclase complex on the differentiating erythroid cell and to elucidate its role in the complex interplay of erythropoietin and other hormonal effects during erythropoiesis.
我们已在促红细胞生成素反应性细胞系劳舍尔小鼠红白血病中展示了红系分化的形态学和生化证据。这些细胞在促红细胞生成素和二甲基亚砜的作用下合成成人血红蛋白以及可能的胚胎血红蛋白。在不同诱导剂作用下，一些克隆之间观察到明显差异。这些差异的分子基础可能与诱导剂作用的不同模式有关，必须加以阐明。这些细胞拥有一种功能性β - 肾上腺素能受体/腺苷酸环化酶复合物，与在几个物种的红细胞上发现的复合物非常相似。受体密度由促红细胞生成素特异性上调，因此，这是该激素对膜受体调节的首次描述。因此，我们现在有机会研究分化中的红系细胞上受体 - 环化酶复合物的发育，并阐明其在红细胞生成过程中促红细胞生成素和其他激素效应的复杂相互作用中的作用。
