Skucas J, Gluckman J B, Fowler E H, Turner M D, Narisawa T
Invest Radiol. 1978 Jan-Feb;13(1):34-9. doi: 10.1097/00004424-197801000-00008.
Colon carcinomata were induced in rats with N-methyl-N-nitro-N-nitrosoguanidine. A magnified double contrast barium enema technique was developed for an in vivo study of tumor growth patterns. Invariably, all small tumors were smoothly outlined and were spherical or ellipsoidal in shape. Irregularity developed only when the tumors grew larger, at which time there was considerable variation in the tumor growth patterns. The onset, shape, progression, spread and number of tumors present can be studied without sacrificing the animal.
用N-甲基-N-硝基-N-亚硝基胍在大鼠体内诱发结肠癌。开发了一种放大的双对比钡灌肠技术用于肿瘤生长模式的体内研究。无一例外，所有小肿瘤都轮廓光滑，呈球形或椭圆形。只有当肿瘤变大时才会出现不规则，此时肿瘤生长模式存在相当大的差异。可以在不牺牲动物的情况下研究肿瘤的发生、形状、进展、扩散和数量。
