Clauser E, Bouhnik J, Gonzalez M F, Corvol P, Menard J
Am J Physiol. 1984 Feb;246(2 Pt 1):E129-33. doi: 10.1152/ajpendo.1984.246.2.E129.
The effects of high plasma renin levels on plasma levels of both total immunoreactive angiotensinogen (direct radioimmunoassay) and intact angiotensinogen measured by angiotensin I released by renin (indirect assay) were studied in sodium-depleted rats both with and without captopril treatment and in adrenalectomized rats. The direct assay measures both intact angiotensinogen and des-angiotensin I-angiotensinogen, its residue cleaved by renin. The indirect assay measures only intact angiotensinogen. Neither sodium depletion, captopril treatment, nor adrenalectomy modified the circulating levels of total angiotensinogen. However these treatments produced a decrease in intact angiotensinogen that was in proportion to the elevation of renin levels. The difference between the two assays for angiotensin represents the level of des-angiotensin I-angiotensinogen and correlated satisfactorily with the plasma levels of renin. Identical correlations were observed in adrenalectomized rats and captopril-treated rats. We conclude that des-angiotensin I-angiotensinogen levels are an index of activation of the renin-angiotensin system dependent on the circulating level of renin.
在钠缺乏的大鼠中，无论是否接受卡托普利治疗，以及在肾上腺切除的大鼠中，研究了高血浆肾素水平对总免疫反应性血管紧张素原（直接放射免疫测定法）和通过肾素释放的血管紧张素I测量的完整血管紧张素原（间接测定法）的血浆水平的影响。直接测定法测量完整的血管紧张素原和去血管紧张素I-血管紧张素原（其为肾素裂解后的残余物）。间接测定法仅测量完整的血管紧张素原。钠缺乏、卡托普利治疗或肾上腺切除术均未改变总血管紧张素原的循环水平。然而，这些治疗导致完整血管紧张素原减少，且与肾素水平的升高成比例。两种血管紧张素测定法之间的差异代表去血管紧张素I-血管紧张素原的水平，并与血浆肾素水平具有良好的相关性。在肾上腺切除的大鼠和卡托普利治疗的大鼠中观察到相同的相关性。我们得出结论，去血管紧张素I-血管紧张素原水平是依赖于循环肾素水平的肾素-血管紧张素系统激活的一个指标。
