环磷酸腺苷（cAMP）、呋塞米和哇巴因对大鼠经气管四环素和氯霉素内流的影响。

Effects of cAMP, furosemide, and ouabain on transtracheal tetracycline and chloramphenicol influx in the rat.

作者信息

Cloutier M M, Lesniak K M

出版信息

Am Rev Respir Dis. 1984 Feb;129(2):284-7.

PMID:6320697

Abstract

Using an in vitro perfusion model of the rat trachea, we studied the effects of dibutyryl cAMP (10(-3)M), terbutaline sulfate (1.7 X 10(-4)M), furosemide (10(-3)M), and ouabain (10(-3)M) on net transtracheal tetracycline and chloramphenicol influx. Submucosal cAMP and terbutaline increased transepithelial potential difference and short-circuit current and net tetracycline influx from control values of 0.496 +/- 0.066 (mean +/- SEM, n = 9) and 0.451 +/- 0.077 nM/cm2 h (n = 7) by 51 and 29%, respectively. Submucosal furosemide and ouabain decreased transepithelial potential difference, short-circuit current, and net tetracycline influx from control values by 22 and 29%, respectively. Neither cAMP, terbutaline, furosemide, nor ouabain had any effect on net chloramphenicol influx. These data suggest that drugs used in conjunction with antibiotics in patients with obstructive pulmonary disease may alter delivery of certain antibiotics into the airway lumen.

摘要

利用大鼠气管的体外灌注模型，我们研究了二丁酰环磷腺苷（10⁻³M）、硫酸特布他林（1.7×10⁻⁴M）、呋塞米（10⁻³M）和哇巴因（10⁻³M）对气管四环素和氯霉素净内流的影响。黏膜下环磷腺苷和特布他林使跨上皮电位差、短路电流以及四环素净内流分别从对照值0.496±0.066（平均值±标准误，n = 9）和0.451±0.077 nM/cm²·h（n = 7）增加了51%和29%。黏膜下呋塞米和哇巴因使跨上皮电位差、短路电流以及四环素净内流分别从对照值降低了22%和29%。环磷腺苷、特布他林、呋塞米和哇巴因均对氯霉素净内流无任何影响。这些数据表明，在阻塞性肺病患者中与抗生素联合使用的药物可能会改变某些抗生素向气道管腔的输送。