Gilboe D P, Nuttall F Q
Arch Biochem Biophys. 1984 Feb 1;228(2):587-91. doi: 10.1016/0003-9861(84)90026-2.
In glycogen particle suspensions prepared from fed rats given either glucagon or glucose in order to increase or decrease the phosphorylase a concentration, respectively, glucose stimulation of synthase phosphatase activity was observed. In preparations from glucagon-treated rats, addition of glucose stimulated synthase and phosphorylase phosphatase simultaneously and not sequentially. Synthase phosphatase stimulation was glucose concentration dependent even when phosphorylase a had been rapidly reduced to a low level. The estimated A0.5 for glucose stimulation of synthase phosphatase activity was 27 mM. An A0.5 for glucose stimulation of phosphorylase phosphatase activity could not be estimated since activity was still increasing with concentrations of glucose as high as 200 mM. In preparations from glucose-treated rats which contain virtually no phosphorylase a, glucose stimulation was still apparent but the A0.5 was increased modestly (36 mM). Stimulation of synthase phosphatase activity was specific for glucose. Several other monosaccharides and the polyhydric alcohol sorbitol were ineffective.
在分别给予胰高血糖素或葡萄糖以增加或降低磷酸化酶a浓度的喂食大鼠制备的糖原颗粒悬浮液中，观察到了葡萄糖对合酶磷酸酶活性的刺激作用。在胰高血糖素处理大鼠的制备物中，添加葡萄糖同时刺激合酶和磷酸化酶磷酸酶，而非顺序刺激。即使磷酸化酶a已迅速降至低水平，合酶磷酸酶的刺激仍依赖于葡萄糖浓度。葡萄糖刺激合酶磷酸酶活性的估计半数效应浓度（A0.5）为27 mM。由于在高达200 mM的葡萄糖浓度下活性仍在增加，因此无法估计葡萄糖刺激磷酸化酶磷酸酶活性的A0.5。在几乎不含磷酸化酶a的葡萄糖处理大鼠的制备物中，葡萄糖刺激仍然明显，但A0.5适度增加（36 mM）。合酶磷酸酶活性的刺激对葡萄糖具有特异性。其他几种单糖和多元醇山梨醇无效。
