利用重组DNA质粒测定培养的哺乳动物细胞中的DNA修复与重组
The use of recombinant DNA plasmids for the determination of DNA-repair and recombination in cultured mammalian cells.
作者信息
Cox R, Masson W K, Debenham P G, Webb M B
出版信息
Br J Cancer Suppl. 1984;6:67-72.
Abstract
Using the recombinant plasmid pSV2gpt and DNA transfer techniques, cell mediated DNA ligation and recombination of plasmid DNA have been demonstrated in four human cell lines. Data suggesting the involvement of a possible defect in the cellular equilibrium between ligation and exonuclease digestion of double strand DNA scissions in an ataxia-telangiectasia (A-T) cell line is discussed. The same A-T line was grossly proficient in DNA recombination but it will be necessary to distinguish between recombination in coding and non-coding plasmid sequences.
摘要
利用重组质粒pSV2gpt和DNA转移技术，已在四种人类细胞系中证实了细胞介导的质粒DNA连接和重组。讨论了有关共济失调毛细血管扩张症（A-T）细胞系中双链DNA断裂连接与核酸外切酶消化之间细胞平衡可能存在缺陷的数据。同一A-T细胞系在DNA重组方面总体上是熟练的，但有必要区分编码和非编码质粒序列中的重组。
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