聚乙二醇（PEG 600和PEG 6000）及牛血清白蛋白对体外髓过氧化物酶介导的大鼠腹膜肥大细胞释放[3H] - 5 -羟色胺的影响

Effect of polyethyleneglycols (PEG 600 and PEG 6000) and bovine serum albumin on myeloperoxidase-mediated release of [3H]-serotonin from rat peritoneal mast cells in vitro.

作者信息

Magnusson K E, Stendahl O

出版信息

Biosci Rep. 1984 Jan;4(1):33-7. doi: 10.1007/BF01120821.

DOI:10.1007/BF01120821

PMID:6320925

Abstract

Rat peritoneal mast cells in vitro released [3H]-serotonin in the presence of halide (I-), myeloperoxidase (MPO), and a H2O2-generating system. The degranulation was partly inhibited with low concentrations of bovine serum albumin (BSA; 0.1-1% w/v), or polyethyleneglycols (PEG 600 or PEG 6000, 0.1% w/v). Furthermore, PEG 600 and PEG 6000 enhanced the stabilization achieved with 0.1% BSA. The results may provide a new aspect on antigenicity of PEG-modified allergens, and on mast-cell response at an inflammatory site.

摘要

在卤化物（I-）、髓过氧化物酶（MPO）和一个产生H2O2的系统存在的情况下，体外培养的大鼠腹膜肥大细胞释放出[3H] - 5 - 羟色胺。低浓度的牛血清白蛋白（BSA；0.1 - 1% w/v）或聚乙二醇（PEG 600或PEG 6000，0.1% w/v）可部分抑制脱颗粒作用。此外，PEG 600和PEG 6000增强了用0.1% BSA所实现的稳定作用。这些结果可能为聚乙二醇修饰的过敏原的抗原性以及炎症部位肥大细胞的反应提供一个新的视角。