通过沉淀冷冻溶解法对无机离子进行定位

Localization of inorganic ions by precipitative freeze dissolution.

作者信息

van Iren F, Bange G G

出版信息

Histochemistry. 1978 Mar 2;55(2):81-96. doi: 10.1007/BF00493511.

Abstract

A new method for localization of inorganic diffusible ions in tissue is introduced. It has been applied to localization of Tl+ and Rb+ in barley roots and is probably also suited for Cs+, Ca2+, Cl-, Br-, PO 3/4- and perhaps K+. Its principle consists of dissolution of the ice from frozen tissue in a concentrated aqueous solution of a precipitating agent that is kept at a temperature just above its melting point.

摘要

介绍了一种用于组织中无机扩散离子定位的新方法。它已应用于大麦根中铊离子（Tl+）和铷离子（Rb+）的定位，可能也适用于铯离子（Cs+）、钙离子（Ca2+）、氯离子（Cl-）、溴离子（Br-）、磷酸根离子（PO₄³⁻），或许还有钾离子（K+）。其原理是在略高于熔点的温度下，将冷冻组织中的冰溶解在沉淀剂的浓水溶液中。