强化牛奶中维生素A和D的反相液相色谱测定方法的改进

Improved reverse phase liquid chromatographic determination of vitamins A and D in fortified milk.

作者信息

Wickroski A F, McLean L A

出版信息

J Assoc Off Anal Chem. 1984 Jan-Feb;67(1):62-5.

Abstract

A method for determination of vitamins A, D2, and D3 on the same 6 mL sample of fortified milk is described. A special indicator permits selection from the cleanup column of a fraction that contains all vitamin D and is free of cholesterol interference for final measurement by reverse phase liquid chromatography. Conservation of analyst's time and expensive solvents results from the relatively small sample processed. The average recoveries and standard deviations were, respectively, 94.1% and 4.8 for vitamin D2, 91.1% and 5.2 for vitamin D3, and 92.2% and 2.6 for vitamin A in nonfat skim milk; 86.5% and 9.4 for vitamin D2, and 83.7% and 4.9 for vitamin D3 in homogenized whole milk. Detectable minima are about 100 IU vitamin A/qt and 50 IU vitamin D2 or D3/qt.

摘要

本文描述了一种在6毫升强化牛奶样品中同时测定维生素A、D2和D3的方法。一种特殊的指示剂可从净化柱中选择含有所有维生素D且无胆固醇干扰的馏分，以便通过反相液相色谱法进行最终测定。由于处理的样品量相对较小，节省了分析人员的时间和昂贵的溶剂。在脱脂牛奶中，维生素D2的平均回收率和标准偏差分别为94.1%和4.8，维生素D3为91.1%和5.2，维生素A为92.2%和2.6；在全脂均质牛奶中，维生素D2为86.5%和9.4，维生素D3为83.7%和4.9。可检测的最小值约为每夸脱100国际单位维生素A和每夸脱50国际单位维生素D2或D3。