Faulkner H, Hussein A, Foran M, Szijarto L
Laboratory Services Division, University of Guelph, ON, Canada.
J Dairy Sci. 2000 Jun;83(6):1210-6. doi: 10.3168/jds.S0022-0302(00)74986-1.
High performance liquid chromatographic methods for measuring the concentration of vitamins A and D in fluid milk were validated and used to assess the level of these nutrients in Ontario retail milk samples. Thirteen and fifteen fortified milk samples were tested for vitamins A and D, respectively. Repeatability relative standard deviation values for vitamins A and D in milk were generally less than 10%. Recoveries varied from 87 to 107%. Vitamin D results indicated that only 20% of skim, 40% of 2% fat milk, and 20% of whole milk contained the recommended levels, whereas 46% of skim, and 77% of 2% fat milk had the required levels of vitamin A. The results indicate that vitamin level varies widely in Ontario retail milk.
用于测定液态奶中维生素A和D浓度的高效液相色谱法经过验证，并用于评估安大略省零售牛奶样本中这些营养素的水平。分别对13个和15个强化牛奶样本进行了维生素A和D的检测。牛奶中维生素A和D的重复性相对标准偏差值通常小于10%。回收率在87%至107%之间。维生素D的检测结果表明，只有20%的脱脂牛奶、40%的2%脂肪牛奶和20%的全脂牛奶含有推荐水平的维生素D，而46%的脱脂牛奶和77%的2%脂肪牛奶含有所需水平的维生素A。结果表明，安大略省零售牛奶中的维生素水平差异很大。
