Tokunaga K, Hirose S, Suzuki Y
Nucleic Acids Res. 1984 Feb 10;12(3):1543-58. doi: 10.1093/nar/12.3.1543.
The DNA sequences necessary for faithful and efficient initiation of transcription of Bombyx mori fibroin gene have been studied in vivo using monkey COS cells and a SV40 origin vector. Transcriptional analysis of 5' deletion genes and of exact substitution genes including a series of single base substitution mutants indicates that the TATA box and the cap region are required for faithful and efficient initiation of the fibroin gene transcription in vivo, whereas far upstream sequences and the CAAT-like sequences are not required. In addition, single base substitution genes of the cap site region have clearly shown that there exists a strict sequence requirement of the cap region of the fibroin gene. These results define the basic promoter of the fibroin gene to the region spanning the TATA box and the cap site region.
利用猴COS细胞和SV40起始载体在体内研究了家蚕丝心蛋白基因忠实高效转录起始所需的DNA序列。对5'缺失基因以及包括一系列单碱基取代突变体在内的精确取代基因进行转录分析表明，TATA框和帽区是家蚕丝心蛋白基因在体内忠实高效转录起始所必需的，而远上游序列和类CAAT序列则不是必需的。此外，帽位点区域的单碱基取代基因清楚地表明，丝心蛋白基因的帽区存在严格的序列要求。这些结果将丝心蛋白基因的基本启动子定义为跨越TATA框和帽位点区域的区域。
