Zagrebel'nyĭ S N, Zernov Iu P, Maĭstrenko V F, Pustoshilova N M
Prikl Biokhim Mikrobiol. 1984 Jan-Feb;20(1):24-30.
A technique for isolation of RNA-ligase of bacteriophage T4 was proposed. It is mainly based on the using of Soviet materials and sorbents and includes seven purification stages. The technique enables to isolate about 80 000 units of active enzyme from 100 g of E. coli B cells infected with the phage. T4am N82; that makes up 20% of the activity of the cell extract. The obtained preparations of RNA-ligase are homogeneous by the data of electrophoresis and practically, free of endo- and exonuclease admixtures.
提出了一种分离噬菌体T4 RNA连接酶的技术。该技术主要基于苏联材料和吸附剂的使用，包括七个纯化阶段。该技术能够从100克感染噬菌体T4am N82的大肠杆菌B细胞中分离出约80000个活性酶单位，这占细胞提取物活性的20%。通过电泳数据可知，所获得的RNA连接酶制剂是均一的，并且实际上不含内切核酸酶和外切核酸酶杂质。
