Mal'khanov V B, Gripas' I A, Kurenkova T N
Vopr Virusol. 1983 Nov-Dec;28(6):684-6.
The duration and pattern of the course of herpes virus infection of the eye and the method used were shown to influence the effectiveness of the laboratory diagnosis. In the first two weeks of the disease conjunctival scrappings should be examined by immunofluorescence and virus isolation by intracerebral inoculation of mice, at later periods by immunofluorescence of conjunctival scrappings or infected cell cultures. Isolated herpes uveitis should be diagnosed by demonstration of viremia. In surgical treatment of herpetic eye diseases virus should be demasked from the cornea by cocultivation of its fragments with human embryo fibroblast culture.
眼部疱疹病毒感染病程的持续时间和模式以及所采用的方法被证明会影响实验室诊断的有效性。在疾病的头两周,应通过免疫荧光检查结膜刮片,并通过小鼠脑内接种进行病毒分离,在后期则通过结膜刮片或感染细胞培养的免疫荧光检查。孤立性疱疹性葡萄膜炎应通过证实病毒血症来诊断。在疱疹性眼病的外科治疗中,应通过将角膜碎片与人胚成纤维细胞培养物共培养来从角膜中揭示病毒。