Pak H Y, Teplitz R L, Ashdjian V, Yokota S B, Hammond W G, Benfield J R
Anal Quant Cytol. 1983 Dec;5(4):263-8.
Comparative DNA measurements in human and canine preneoplastic and neoplastic tracheobronchial cells were made with the application of computerized image analysis. Canine studies demonstrated that the sequence of cellular events that precede epidermoid lung cancer simulates precisely the progression observed in humans. DNA studies concomitantly confirmed that there is a stepwise increase in DNA content with advancing nuclear atypia in metaplastic respiratory cells in both species. All carcinomas, regardless of histologic type, were significantly hyperploid to aneuploid (4c to 6c). Small-cell carcinoma exhibited a narrow modal distribution in the 4c region. The uniformity of the cytologic and quantitative DNA changes among these disparate species tends to confirm that humans and canines share biologic characteristics in bronchogenic carcinogenesis. The quantitative DNA measurements provide an objective cellular marker and may be used clinically for diagnostic purposes.
运用计算机图像分析技术对人类和犬类的肿瘤前及肿瘤性气管支气管细胞进行了DNA对比测量。犬类研究表明，在肺表皮样癌之前发生的细胞事件序列与人类所观察到的进展过程精确模拟。DNA研究同时证实，在这两个物种的化生呼吸细胞中，随着核异型性增加，DNA含量呈逐步上升。所有癌，无论组织学类型如何，均显著超倍体至非整倍体（4c至6c）。小细胞癌在4c区域呈现狭窄的众数分布。这些不同物种间细胞学和定量DNA变化的一致性倾向于证实，人类和犬类在支气管源性致癌过程中具有共同的生物学特征。定量DNA测量提供了一个客观的细胞标志物，可在临床上用于诊断目的。
