Berliner L J
Ann N Y Acad Sci. 1983;414:153-61. doi: 10.1111/j.1749-6632.1983.tb31681.x.
Labeling experiments with viable membrane preparations are optimally undertaken where conditions are extremely mild (minimal excess of modification reagent, very short reaction times) in order to reduce the possibilities of introducing artifacts (cell aging and death, denaturation, etc.). Furthermore, specificity of labeling is a critical requirement where the system is complex and contains many proteins. The spin label IV described above offers several advantages over previously employed reagents. By virtue of its reversibility and ease of quantitation, one may repetitively label and remove label from the same membrane preparation in order to check reproducibility while using the same sample as a control.
使用活细胞膜制剂进行标记实验时，最好在极其温和的条件下进行（修饰试剂过量极少，反应时间极短），以减少引入假象（细胞老化和死亡、变性等）的可能性。此外，在系统复杂且包含多种蛋白质的情况下，标记的特异性是一项关键要求。上述自旋标记IV与先前使用的试剂相比具有几个优点。由于其可逆性和易于定量，人们可以对同一膜制剂重复进行标记和去除标记，以便在使用相同样品作为对照时检查重现性。
