Girotti A W, Thomas J P
Biochem Biophys Res Commun. 1984 Jan 30;118(2):474-80. doi: 10.1016/0006-291x(84)91327-5.
Isolated erythrocyte membranes incubated with xanthine, xanthine oxidase, and Fe(III) underwent lipid peroxidation, as indicated by the thiobarbituric acid reaction and iodometric determination of hydroperoxides. In detergent-free medium (phosphate buffered saline) peroxidation was inhibited by superoxide dismutase, catalase, and EDTA; but was promoted by OH. scavangers, eg. mannitol. Generation of OH. in the system via iron-catalyzed reduction of H2O2 by O-2 was demonstrated by EPR spectrometry using spin trapping. In membranes treated with Triton X-100 lipid peroxidation was stimulated by EDTA and suppressed by OH. traps. This and other evidence suggests that OH. in the medium was an effective initiator of lipid peroxidation in detergent-dispersed membranes, but not in intact membranes.
用黄嘌呤、黄嘌呤氧化酶和Fe(III)孵育的分离红细胞膜发生了脂质过氧化，这通过硫代巴比妥酸反应和氢过氧化物的碘量法测定得以表明。在无去污剂的介质（磷酸盐缓冲盐水）中，超氧化物歧化酶、过氧化氢酶和EDTA可抑制过氧化；但OH.清除剂，如甘露醇，则会促进过氧化。通过自旋捕获的电子顺磁共振光谱法证明，该体系中通过铁催化O-2还原H2O2产生了OH.。在用Triton X-100处理的膜中，EDTA刺激脂质过氧化，而OH.捕获剂则抑制脂质过氧化。这一现象及其他证据表明，介质中的OH.是去污剂分散膜中脂质过氧化的有效引发剂，但在完整膜中并非如此。
