降钙素与钙调蛋白的体外相互作用。

In vitro interaction between calcitonin and calmodulin.

作者信息

Gnessi L, Camilloni G, Fabbri A, Politi V, DeLuca G, DiStazio G, Moretti C, Fraioli F

出版信息

Biochem Biophys Res Commun. 1984 Jan 30;118(2):648-54. doi: 10.1016/0006-291x(84)91352-4.

Abstract

The effects of salmon calcitonin and human calcitonin on the fluorescence spectra of a fluorescent conjugate of calmodulin with dansyl-chloride and on the calmodulin mediated activation of phosphodiesterase have been studied. We showed that salmon calcitonin provoked calcium-dependent modifications of the spectra of dansyl-calmodulin and completely inhibited the calmodulin mediated activation of phosphodiesterase with a maximum effect at the dose of 5.8 X 10(-7)M and 10(-7) M respectively. No appreciable effect of human calcitonin was observed in either system. This interaction of salmon calcitonin with calmodulin is similar to the interaction of certain neuropeptides and antipsychotic drugs to calmodulin and may explain certain of the peptide's pharmacological effects.

摘要

研究了鲑鱼降钙素和人降钙素对钙调蛋白与丹磺酰氯荧光结合物荧光光谱以及对钙调蛋白介导的磷酸二酯酶激活的影响。我们发现，鲑鱼降钙素可引起丹磺酰钙调蛋白光谱的钙依赖性修饰，并完全抑制钙调蛋白介导的磷酸二酯酶激活，分别在剂量为5.8×10⁻⁷M和10⁻⁷M时效果最佳。在任一系统中均未观察到人降钙素的明显作用。鲑鱼降钙素与钙调蛋白的这种相互作用类似于某些神经肽和抗精神病药物与钙调蛋白的相互作用，这可能解释了该肽的某些药理作用。