Louters L, Chalkley R
Biochemistry. 1984 Jan 31;23(3):547-52. doi: 10.1021/bi00298a023.
We have asked whether exogenous, radiolabeled histones can exchange with nucleosomal histones in an in vitro system. Using two different electrophoretic techniques, we were able to separate the histones contained in nucleosomes from those histones which were simply bound to the surface of the chromatin. Fluorography was used to determine which of the exogenous histones exchange with the nucleosomal histones. We observed substantial exchange of histones H1, H2a, and H2b when the chromatin and exogenous histones were incubated under approximately physiological conditions. We have also observed a small amount of exchange of H2a and H2b, as well as a substantial exchange of H1, from one chromatin fragment to another. Other conditions affecting the exchange of histones H2a and H2b are also reported.
我们曾探讨外源性放射性标记组蛋白能否在体外系统中与核小体组蛋白发生交换。运用两种不同的电泳技术，我们得以将核小体中所含的组蛋白与那些仅结合在染色质表面的组蛋白区分开来。采用荧光自显影法来确定哪些外源性组蛋白会与核小体组蛋白发生交换。当染色质与外源性组蛋白在近似生理条件下孵育时，我们观察到组蛋白H1、H2a和H2b发生了大量交换。我们还观察到H2a和H2b有少量交换，以及H1从一个染色质片段到另一个染色质片段的大量交换。本文还报道了影响组蛋白H2a和H2b交换的其他条件。
