• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

含有二硝基苯基化组蛋白的核小体的重建。

Reconstitution of nucleosomes containing dinitrophenylated histones.

作者信息

MacDonald H S, Herman T M

出版信息

Biochem J. 1985 Apr 1;227(1):125-8. doi: 10.1042/bj2270125.

DOI:10.1042/bj2270125
PMID:3994677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1144816/
Abstract

Histone octamers of purified monomer nucleosomes were labelled with [3H]dinitrofluorobenzene. Authentic 11 S nucleosomes were reconstituted in vitro from a mixture of [3H]dinitrophenylated histones and excess unlabelled monomer nucleosomes. The reconstituted nucleosomes were found to contain [3H]dinitrophenylated histones H2a and H2b but not [3H]dinitrophenylated histones H3 and H4. Approx. 83% of [3H]dinitrophenylated nucleosomes were immunoprecipitable with anti-dinitrophenyl immunoglobulin and Staphylococcus aureus. These results demonstrate that histones H2a and H2b contain dinitrofluorobenzene-reactive groups that can be modified without destroying their ability to participate in nucleosome formation in vitro.

摘要

纯化的单体核小体的组蛋白八聚体用[3H]二硝基氟苯进行标记。从[3H]二硝基苯基化组蛋白和过量未标记的单体核小体的混合物中体外重建真实的11S核小体。发现重建的核小体含有[3H]二硝基苯基化的组蛋白H2a和H2b，但不含有[3H]二硝基苯基化的组蛋白H3和H4。约83%的[3H]二硝基苯基化核小体可用抗二硝基苯基免疫球蛋白和金黄色葡萄球菌进行免疫沉淀。这些结果表明，组蛋白H2a和H2b含有可被二硝基氟苯修饰的反应基团，且修饰后不破坏它们在体外参与核小体形成的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/926e/1144816/60a2b8a8c86b/biochemj00306-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/926e/1144816/60a2b8a8c86b/biochemj00306-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/926e/1144816/60a2b8a8c86b/biochemj00306-0131-a.jpg

相似文献

1
Reconstitution of nucleosomes containing dinitrophenylated histones.含有二硝基苯基化组蛋白的核小体的重建。
Biochem J. 1985 Apr 1;227(1):125-8. doi: 10.1042/bj2270125.
2
Binding mode of nucleosome-assembly protein (AP-I) and histones.核小体组装蛋白（AP-I）与组蛋白的结合模式。
Eur J Biochem. 1987 Jan 2;162(1):19-24. doi: 10.1111/j.1432-1033.1987.tb10535.x.
3
Different mechanism for in vitro formation of nucleosome core particles.体外形成核小体核心颗粒的不同机制。
Biochemistry. 1991 May 21;30(20):5022-32. doi: 10.1021/bi00234a026.
4
Histone compostion of chromatin subunits studied by immunosedimentation.通过免疫沉淀法研究染色质亚基的组蛋白组成。
Biochemistry. 1976 Sep 21;15(19):4305-12. doi: 10.1021/bi00664a026.
5
Deposition of newly synthesized histones: new histones H2A and H2B do not deposit in the same nucleosome with new histones H3 and H4.新合成组蛋白的沉积：新的H2A和H2B组蛋白不会与新的H3和H4组蛋白沉积在同一个核小体中。
Biochemistry. 1987 Apr 21;26(8):2315-25. doi: 10.1021/bi00382a037.
6
The histone H3/H4.N1 complex supplemented with histone H2A-H2B dimers and DNA topoisomerase I forms nucleosomes on circular DNA under physiological conditions.在生理条件下，补充了组蛋白H2A-H2B二聚体和DNA拓扑异构酶I的组蛋白H3/H4.N1复合物在环状DNA上形成核小体。
J Biol Chem. 1990 Aug 25;265(24):14487-96.
7
Histone release during transcription: NAP1 forms a complex with H2A and H2B and facilitates a topologically dependent release of H3 and H4 from the nucleosome.转录过程中的组蛋白释放：核小体组装蛋白1（NAP1）与H2A和H2B形成复合物，并促进H3和H4从核小体中进行拓扑依赖性释放。
Biochemistry. 2004 Mar 9;43(9):2359-72. doi: 10.1021/bi035737q.
8
Fractionation of newly replicated nucleosomes by density labeling and rate zonal centrifugation for the analysis of the deposition sites of newly synthesized nucleosomal core histones.通过密度标记和速率区带离心对新复制的核小体进行分级分离，以分析新合成的核小体核心组蛋白的沉积位点。
Eur J Biochem. 1985 Aug 1;150(3):575-80. doi: 10.1111/j.1432-1033.1985.tb09059.x.
9
Effects of histone acetylation, ubiquitination and variants on nucleosome stability.组蛋白乙酰化、泛素化及变体对核小体稳定性的影响。
Biochem J. 1993 Dec 15;296 ( Pt 3)(Pt 3):737-44. doi: 10.1042/bj2960737.
10
Rearrangement of nucleosomal components by modification of histone amino groups. Structural role of lysine residues.
Biochemistry. 1984 Sep 11;23(19):4280-4. doi: 10.1021/bi00314a004.

本文引用的文献

1
Nucleosome structure.核小体结构。
Annu Rev Biochem. 1980;49:1115-56. doi: 10.1146/annurev.bi.49.070180.005343.
2
Tritium labeling of proteins to high specific radioactivity by reduction methylation.通过还原甲基化将蛋白质标记至高比放射性的氚标记法。
J Biol Chem. 1980 Sep 25;255(18):8842-7.
3
In vitro exchange of nucleosomal histones H2a and H2b.核小体组蛋白H2a和H2b的体外交换
Biochemistry. 1984 Jan 31;23(3):547-52. doi: 10.1021/bi00298a023.
4
A reevaluation of new histone deposition on replicating chromatin.对复制染色质上新组蛋白沉积的重新评估。
J Biol Chem. 1981 May 25;256(10):5095-103.
5
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中氚标记蛋白质和核酸的胶片检测方法。
Eur J Biochem. 1974 Jul 1;46(1):83-8. doi: 10.1111/j.1432-1033.1974.tb03599.x.
6
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.用葡萄球菌蛋白A-抗体吸附剂从细胞中快速分离抗原：抗体-抗原复合物与蛋白A相互作用的参数
J Immunol. 1975 Dec;115(6):1617-24.
7
Analysis of subunit organization in chicken erythrocyte chromatin.鸡红细胞染色质中亚基组织的分析。
Proc Natl Acad Sci U S A. 1976 Feb;73(2):505-9. doi: 10.1073/pnas.73.2.505.
8
The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.染色质中组蛋白与DNA的组织形式：富含精氨酸的组蛋白核心的证据。
Cell. 1976 Jul;8(3):333-47. doi: 10.1016/0092-8674(76)90145-8.
9
Reconstitution of chromatin core particles.染色质核心颗粒的重建。
Biochemistry. 1977 Nov 29;16(24):5295-303. doi: 10.1021/bi00643a021.
10
The histone core complex: an octamer assembled by two sets of protein-protein interactions.组蛋白核心复合体：一种由两组蛋白质 - 蛋白质相互作用组装而成的八聚体。
Biochemistry. 1978 Nov 14;17(23):4955-64. doi: 10.1021/bi00616a016.