Pincus J H, Weinfeld H M
Brain Res. 1984 Apr 2;296(2):313-7. doi: 10.1016/0006-8993(84)90067-2.
The release of labeled acetylcholine from synaptosomes loaded with methyl-[3H]choline has been measured in Krebs-Ringer-Bicarbonate (KRB) media containing either 5.6 or 56 mM KCl. Experiments have been performed in media containing either 1.0 mM Ca or 0 Ca with 1 mM EGTA. Phenytoin, 2 X 10(-4) M, reduced the depolarization-dependent release of acetylcholine in media containing 1.0 mM Ca and 56 mM KCl. It also significantly increased the release of acetylcholine from undepolarized samples in 5.6 mM KCl irrespective of the Ca concentration. The drug did not affect release from synaptosomes depolarized in Ca-free media. These results confirm the hypothesis that phenytoin has a dual effect on transmitter release.
已在含有5.6或56 mM氯化钾的 Krebs-林格碳酸氢盐（KRB）培养基中测量了从负载甲基-[3H]胆碱的突触体中释放标记的乙酰胆碱的情况。实验是在含有1.0 mM钙或含1 mM乙二醇双乙醚二胺四乙酸（EGTA）的无钙培养基中进行的。2×10⁻⁴ M的苯妥英降低了含有1.0 mM钙和56 mM氯化钾的培养基中乙酰胆碱的去极化依赖性释放。它还显著增加了在5.6 mM氯化钾中未去极化样品中乙酰胆碱的释放，而与钙浓度无关。该药物不影响在无钙培养基中去极化的突触体的释放。这些结果证实了苯妥英对递质释放具有双重作用的假设。
