Korchak H M, Vienne K, Rutherford L E, Wilkenfeld C, Finkelstein M C, Weissmann G
J Biol Chem. 1984 Apr 10;259(7):4076-82.
The role of changes in cytosolic free calcium (Ca) in the activation sequence of the human neutrophil has been monitored by means of the fluorescent probe Quin2. "Complete" secretagogues such as the chemotactic peptide f-Met-Leu-Phe and aggregated IgG, as well as the "incomplete" secretagogue concanavalin A, elicited prompt rises in cytosolic Ca. The rise in cytosolic Ca was in all cases one of the earliest measurable events, consistent with the hypothesis that increments in cytosolic free Ca serve as a signal to activate subsequent physiological responses. The source of cytosolic Ca is principally intracellular, since removal of extracellular Ca had little effect on the rise in cytosolic Ca. Although increments in cytosolic Ca may be essential for neutrophil activation, they are evidently not sufficient; chemotactic levels of f-Met-Leu-Phe elicited optimal increments in cytosolic Ca without triggering degranulation, O-2 generation, and aggregation. Some other factor, associated with high levels of receptor occupancy, must be required for secretion. The tumor promoter phorbol myristate acetate was an exceptional stimulus, since it elicited degranulation, aggregation, and O-2 generation without triggering a rise in cytosolic Ca. Finally, an efflux of Ca is initiated which serves to maintain low intracellular levels of Ca.
通过荧光探针喹啉-2监测了胞质游离钙（Ca）变化在人中性粒细胞激活序列中的作用。“完全”促分泌剂，如趋化肽f-甲硫氨酰-亮氨酰-苯丙氨酸和聚集的免疫球蛋白G，以及“不完全”促分泌剂伴刀豆球蛋白A，均可引起胞质Ca迅速升高。在所有情况下，胞质Ca升高都是最早可测量的事件之一，这与胞质游离Ca增加作为激活后续生理反应信号的假说一致。胞质Ca的来源主要是细胞内的，因为去除细胞外Ca对胞质Ca升高影响很小。虽然胞质Ca增加可能对中性粒细胞激活至关重要，但显然并不充分；趋化水平的f-甲硫氨酰-亮氨酰-苯丙氨酸可引起胞质Ca的最佳增加，但不会触发脱颗粒、超氧阴离子生成和聚集。分泌必须需要一些与高受体占有率相关的其他因素。肿瘤启动子佛波酯肉豆蔻酸酯是一种特殊的刺激物，因为它可引起脱颗粒、聚集和超氧阴离子生成，而不会触发胞质Ca升高。最后，启动了Ca外流，以维持细胞内低水平的Ca。
