Müllbacher A, Parish C R, Mundy J P
J Immunol Methods. 1984 Mar 30;68(1-2):205-15. doi: 10.1016/0022-1759(84)90151-0.
An improved colorimetric assay for estimation of cytotoxic T (Tc) cells is described. The method involves staining thioglycollate-induced macrophage targets with the dye neutral red prior to addition of cytotoxic T cells and estimating macrophage survival at the end of the assay by measuring dye remaining in viable targets. The method using macrophage targets is more sensitive than the 51Cr release assay employing macrophages or a variety of other targets. It may be used to detect alloreactive and H-2 restricted Tc cells in both short-term (4 h) and long-term (24 h) assays and overcomes some variability encountered with a previously described colorimetric procedure. Furthermore, the method is cheap, fast, reliable and avoids the use of radioactivity.
本文描述了一种改进的比色法，用于评估细胞毒性T（Tc）细胞。该方法包括在加入细胞毒性T细胞之前，用中性红染料对巯基乙酸诱导的巨噬细胞靶标进行染色，并在测定结束时通过测量存活靶标中剩余的染料来评估巨噬细胞的存活率。使用巨噬细胞靶标的方法比采用巨噬细胞或多种其他靶标的51Cr释放测定法更灵敏。它可用于在短期（4小时）和长期（24小时）测定中检测同种异体反应性和H-2限制性Tc细胞，并克服了先前描述的比色法所遇到的一些变异性。此外，该方法成本低、速度快、可靠，且避免了使用放射性物质。
