Molenaar P C, Polak R L
Neurosci Lett. 1983 Dec 30;43(2-3):209-13. doi: 10.1016/0304-3940(83)90189-1.
Bound and free acetylcholine (ACh) were measured in frog sartorius muscles by mass fragmentography. Upon incubation of the muscles for 5 min with potassium propionate, which stimulated the release of ACh, there was a 2-fold reduction of bound ACh. In contrast, the amount of free ACh remained unchanged. After 65 min recovery from stimulation in normal Ringer solution containing deuterium-labelled choline, free ACh was labelled to a higher degree than bound ACh. The results are in agreement with the idea that ACh is synthesized in the cytoplasmic compartment of the motor nerve terminal, and subsequently transferred to the vesicles from which it is released upon stimulation.
采用质量碎片分析法测定青蛙缝匠肌中结合型和游离型乙酰胆碱（ACh）的含量。用丙酸钾孵育肌肉5分钟，可刺激ACh释放，此时结合型ACh减少了两倍。相比之下，游离型ACh的量保持不变。在含有氘标记胆碱的正常林格液中刺激恢复65分钟后，游离型ACh的标记程度高于结合型ACh。这些结果与以下观点一致：ACh在运动神经末梢的细胞质区室中合成，随后转移到囊泡中，在受到刺激时从囊泡中释放出来。
