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脊髓灰质炎病毒基因组连接蛋白VPg的膜依赖性尿苷酸化

Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.

作者信息

Takegami T, Kuhn R J, Anderson C W, Wimmer E

出版信息

Proc Natl Acad Sci U S A. 1983 Dec;80(24):7447-51. doi: 10.1073/pnas.80.24.7447.

DOI:10.1073/pnas.80.24.7447
PMID:6324172
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389968/
Abstract

A small nucleotidyl-protein has been synthesized in vitro in a membrane fraction of poliovirus-infected HeLa cells. Analyses of the nucleotides and polypeptide have shown that the nucleotidyl-protein is VPg-pUpU: the genome-linked protein of poliovirion RNA covalently bound to the first two 5'-terminal nucleotides of poliovirus RNA. Synthesis of VPg-pUpU in vitro was sensitive to nonionic detergent. We suggest that VPg-pUpU is part of the initiation complex in poliovirus RNA replication in a membranous environment.

摘要

一种小分子核苷酸蛋白已在体外由脊髓灰质炎病毒感染的HeLa细胞的膜组分合成。对核苷酸和多肽的分析表明，该核苷酸蛋白是VPg-pUpU：脊髓灰质炎病毒RNA的基因组连接蛋白，与脊髓灰质炎病毒RNA的前两个5'-末端核苷酸共价结合。体外合成VPg-pUpU对非离子去污剂敏感。我们认为VPg-pUpU是膜环境中脊髓灰质炎病毒RNA复制起始复合物的一部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/28e88498b79b/pnas00650-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/eca9890ceafa/pnas00650-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/bfdcf32165d1/pnas00650-0081-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/ac64ebe6a450/pnas00650-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/5db3c3fc2e0d/pnas00650-0082-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/28e88498b79b/pnas00650-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/eca9890ceafa/pnas00650-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/bfdcf32165d1/pnas00650-0081-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/ac64ebe6a450/pnas00650-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/5db3c3fc2e0d/pnas00650-0082-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b1/389968/28e88498b79b/pnas00650-0084-a.jpg

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Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.脊髓灰质炎病毒基因组连接蛋白VPg的膜依赖性尿苷酸化
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7447-51. doi: 10.1073/pnas.80.24.7447.
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本文引用的文献

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Genome-linked proteins of viruses.病毒的基因组连接蛋白
Cell. 1982 Feb;28(2):199-201. doi: 10.1016/0092-8674(82)90335-x.
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Eukaryotic DNA replication: viral and plasmid model systems.真核生物DNA复制：病毒和质粒模型系统
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Amiloride inhibits the initiation of Coxsackievirus and poliovirus RNA replication by inhibiting VPg uridylylation.阿米洛利通过抑制 VPg 尿苷酸化来抑制柯萨奇病毒和脊髓灰质炎病毒 RNA 的复制。
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The twenty-nine amino acid C-terminal cytoplasmic domain of poliovirus 3AB is critical for nucleic acid chaperone activity.脊髓灰质炎病毒 3AB 的 29 个氨基酸 C 末端细胞质结构域对核酸伴侣活性至关重要。
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Identification of tolerated insertion sites in poliovirus non-structural proteins.鉴定脊髓灰质炎病毒非结构蛋白中的耐受插入位点。
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Conversion of VPg into VPgpUpUOH before and during poliovirus negative-strand RNA synthesis.在脊髓灰质炎病毒负链RNA合成之前及过程中，VPg转化为VPgpUpUOH。
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5
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Virology. 1983 Jul 15;128(1):33-47. doi: 10.1016/0042-6822(83)90316-1.
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Determination of sequences at the capped 5' ends of polyoma virus early region transcripts synthesized in vivo and in vitro demonstrates an unusual microheterogeneity.对在体内和体外合成的多瘤病毒早期区域转录本的5' 端加帽序列的测定显示出一种不寻常的微异质性。
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Anti-VPg antibody inhibition of the poliovirus replicase reaction and production of covalent complexes of VPg-related proteins and RNA.抗VPg抗体对脊髓灰质炎病毒复制酶反应的抑制作用以及VPg相关蛋白与RNA共价复合物的产生。
Cell. 1982 Oct;30(3):745-52. doi: 10.1016/0092-8674(82)90279-3.