溶血性质粒pSU316的物理图谱和功能图谱。

Physical and functional map of the hemolytic plasmid pSU316.

作者信息

Andrés I, Rodriguez J C, Ortiz J M

出版信息

Plasmid. 1984 Jan;11(1):96-8. doi: 10.1016/0147-619x(84)90012-x.

Abstract

A restriction endonuclease analysis of the hemolytic plasmid pSU316 has allowed location of the cleavage sites for the endonucleases BamHI, XbaI, KpnI, Bg/II, Sa/GI, EcoRI, and HindIII. Hybridization experiments between pSU316 and pED100 have shown that the tra region of pSU316 lies in a segment comprising part of Sa/GI fragments S-1 and S-3 and the entire fragment S-4. The positions of other plasmids coded functions, namely the replication functions and alpha-hemolysin production, have been determined in the physical map.

摘要

对溶血性质粒pSU316进行的限制性内切核酸酶分析已确定了内切核酸酶BamHI、XbaI、KpnI、Bg/II、Sa/GI、EcoRI和HindIII的切割位点位置。pSU316与pED100之间的杂交实验表明，pSU316的转移区域位于一个片段中，该片段包括Sa/GI片段S-1和S-3的一部分以及整个片段S-4。在物理图谱中已确定了其他质粒编码功能的位置，即复制功能和α-溶血素的产生。