Bzik D J, Fox B A, DeLuca N A, Person S
Virology. 1984 Mar;133(2):301-14. doi: 10.1016/0042-6822(84)90397-0.
The nucleotide sequence thought to specify the glycoprotein gene, gB, of the KOS strain of herpes simplex virus type 1 (HSV-1) has been determined. A 3.1-kilobase (kb), viral-specified RNA was mapped to the left half of the BamHI-G fragment (0.345 to 0.399 map units). TATA, CAT-box, and possible mRNA start sequences characteristic of HSV-1 genes are found near 0.368 map units. The first available ATG codon is at 0.366 and the first in-phase chain terminator at 0.348 map units. A polyA-addition signal (AATAAA) occurs 17 nucleotides past the chain terminator. Translation of these sequences would yield a 100.3-kilodalton (kDa) polypeptide characterized by a 5' signal sequence, nine N-linked saccharide addition sites, a strongly hydrophobic membrane-spanning sequence, and a highly charged 3' cytoplasmic anchor sequence. Two mutants of KOS, tsJ12 and tsJ20, that are temperature-sensitive for viral growth and for the production of gB, have been physically mapped to 0.357 to 0.360 and 0.360 to 0.364 map units, respectively (DeLuca et al., in preparation). The nucleotide sequence of the mutants was determined in these regions. In both cases a single amino acid replacement within the 100.3-kDa polypeptide is predicted from the sequence analysis.
1型单纯疱疹病毒（HSV-1）KOS株中被认为可指定糖蛋白基因gB的核苷酸序列已被确定。一个3.1千碱基（kb）的病毒特异性RNA被定位到BamHI-G片段的左半部分（0.345至0.399个图距单位）。在0.368个图距单位附近发现了HSV-1基因特有的TATA框、CAT框和可能的mRNA起始序列。第一个可用的ATG密码子位于0.366处，第一个同相位链终止子位于0.348个图距单位处。一个聚腺苷酸添加信号（AATAAA）出现在链终止子之后17个核苷酸处。这些序列的翻译将产生一个100.3千道尔顿（kDa）的多肽，其特征为一个5'信号序列、九个N-连接糖基化添加位点、一个强疏水性跨膜序列和一个高电荷的3'细胞质锚定序列。KOS的两个突变体tsJ12和tsJ20，对病毒生长和gB的产生具有温度敏感性，已分别被物理定位到0.357至0.360和0.360至0.364个图距单位处（DeLuca等人，正在准备中）。在这些区域确定了突变体的核苷酸序列。在这两种情况下，序列分析预测在100.3-kDa多肽内有一个单一氨基酸替换。
