Lerche A, Bisgaard H, Christensen J D, Søndergaard J
Allergy. 1984 Apr;39(3):195-201. doi: 10.1111/j.1398-9995.1984.tb02624.x.
Ten nickel-allergic patients and six healthy control subjects participated in a study of the morphology, kinetics and evolution of the cAMP and cGMP concentrations of migrated leukocytes, using an improved skin chamber technique. Also studied was the effect of nickel exposure in the chamber medium during development of an eczematous reaction in the nickel-allergic patients. Nickel exposure had a specific effect on the morphology, from the 24th hour to the end of the 48 h observation period, with a significant increase in the percentages of basophils, eosinophils and lymphocytes and a decrease of neutrophils. A significantly increased leukocyte migration rate (LMR) was observed from the 27th to 39th hour in six of the allergic patients exposed to nickel. There were no specific permanent changes in cAMP and cGMP concentrations during nickel exposure. The control chambers of the allergic patients and healthy controls had identical leukocyte morphology, LMR and leukocyte concentrations of cAMP and cGMP. However, no correlations were found between LMR, cAMP and cGMP in the eczema patients throughout the observation period.
十名对镍过敏的患者和六名健康对照受试者参与了一项研究，该研究使用改良的皮肤腔室技术，对迁移白细胞中cAMP和cGMP浓度的形态、动力学及演变进行了研究。同时还研究了在镍过敏患者发生湿疹反应过程中，向腔室培养基中添加镍所产生的影响。从第24小时至48小时观察期结束，镍暴露对细胞形态有特定影响，嗜碱性粒细胞、嗜酸性粒细胞和淋巴细胞的百分比显著增加，中性粒细胞减少。在六名接触镍的过敏患者中，从第27小时至39小时观察到白细胞迁移率（LMR）显著增加。镍暴露期间，cAMP和cGMP浓度没有特定的永久性变化。过敏患者和健康对照的对照腔室具有相同的白细胞形态、LMR以及白细胞cAMP和cGMP浓度。然而，在整个观察期内，湿疹患者的LMR、cAMP和cGMP之间未发现相关性。
