钙调蛋白拮抗剂可降低表皮生长因子与转化的人成纤维细胞（而非正常的人成纤维细胞）的结合。
Calmodulin antagonists decrease the binding of epidermal growth factor to transformed, but not to normal, human fibroblasts.
作者信息
Bodine P V, Tupper J T
出版信息
Biochem J. 1984 Mar 1;218(2):629-32. doi: 10.1042/bj2180629.
Abstract
Four psychoactive agents which inhibit calmodulin activity were used to study their effect on the binding of epidermal growth factor (EGF) to normal and simian-virus-40-transformed human fibroblasts (WI38). These calmodulin antagonists decreased the binding of 125I-labelled EGF to the transformed, but not to the normal, cell in a dose-dependent manner. The mechanism of this effect appears to be due to a decrease in the apparent affinity of the plasma-membrane EGF receptor for the EGF molecule.
摘要
使用四种抑制钙调蛋白活性的精神活性药物来研究它们对表皮生长因子（EGF）与正常及猿猴病毒40转化的人成纤维细胞（WI38）结合的影响。这些钙调蛋白拮抗剂以剂量依赖的方式降低了125I标记的EGF与转化细胞而非正常细胞的结合。这种效应的机制似乎是由于质膜EGF受体对EGF分子的表观亲和力降低。
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本文引用的文献
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Related effects of calcium and serum on the G1 phase of the human W138 fibroblast.钙和血清对人W138成纤维细胞G1期的相关影响。
J Cell Physiol. 1980 Jul;104(1):97-103. doi: 10.1002/jcp.1041040113.
2
Can calmodulin inhibitors be used to probe calmodulin effects?钙调蛋白抑制剂可用于探究钙调蛋白的作用吗？
Biochem Pharmacol. 1981 Jul 15;30(14):2031-2. doi: 10.1016/0006-2952(81)90217-3.
3
Determination of the free-energy coupling for binding of calcium ions and troponin I to calmodulin.钙离子与肌钙蛋白I结合至钙调蛋白时自由能偶联的测定。
Biochemistry. 1982 Jan 5;21(1):156-62. doi: 10.1021/bi00530a027.
4
Calmodulin--an intracellular calcium receptor.钙调蛋白——一种细胞内钙受体。
Nature. 1980 May 8;285(5760):73-7. doi: 10.1038/285073a0.
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N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, a calmodulin antagonist, inhibits cell proliferation.N-（6-氨基己基）-5-氯-1-萘磺酰胺，一种钙调蛋白拮抗剂，可抑制细胞增殖。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4354-7. doi: 10.1073/pnas.78.7.4354.
6
Evidence for a role of calmodulin in serum stimulation of Na+ influx in human fibroblasts.钙调蛋白在血清刺激人成纤维细胞钠内流中作用的证据。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3537-41. doi: 10.1073/pnas.79.11.3537.
7
Calcium effects on epidermal growth factor receptor-mediated endocytosis in normal and SV40-transformed human fibroblasts.钙对正常及SV40转化的人成纤维细胞中表皮生长因子受体介导的内吞作用的影响。
J Cell Physiol. 1983 May;115(2):159-66. doi: 10.1002/jcp.1041150209.
8
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