Lev-Ran A, Hwang D, Josefsberg Z, Barseghian G, Kemeny M, Meguid M, Beatty D
Biochem Biophys Res Commun. 1984 Mar 30;119(3):1181-5. doi: 10.1016/0006-291x(84)90900-8.
Microsomes and Golgi fractions were isolated from 13 human liver samples without local malignancy. Binding of insulin to microsomes (per cent per 0.5 mg protein) was 14.4 +/- 7.9% with two classes of receptors: K1 = 1.4 nM, R1 = 0.28 pmol/mg; K2 = 8.1 nM, R2 = 0.62 pmol/mg. The binding was insignificantly lower than in rats. Binding of EGF was only 3.4 +/- 1.7% with two classes of receptors: K1 = 1.4 nM, R1 = 0.06 pmol/mg; K2 = 10.8 nM, R2 = 0.22 pmol/mg; the binding was much lower than in rats (26.3 +/- 5.8%). Binding of insulin to Golgi fraction (per cent per 0.1 mg protein) was 5.5 +/- 0.4% with straight line Scatchard plot; Kd = 5.6 nM, Ro = 3.06 pmol/mg; it was only half of that found in rats. In one case of hepatoma, the binding of insulin to microsomes was normal but that of EGF very low.
从13例无局部恶性病变的人肝脏样本中分离出微粒体和高尔基体组分。胰岛素与微粒体的结合（每0.5毫克蛋白质的百分比）为14.4±7.9%，有两类受体：K1 = 1.4纳摩尔，R1 = 0.28皮摩尔/毫克；K2 = 8.1纳摩尔，R2 = 0.62皮摩尔/毫克。这种结合略低于大鼠。表皮生长因子（EGF）的结合仅为3.4±1.7%，有两类受体：K1 = 1.4纳摩尔，R1 = 0.06皮摩尔/毫克；K2 = 10.8纳摩尔，R2 = 0.22皮摩尔/毫克；这种结合远低于大鼠（26.3±5.8%）。胰岛素与高尔基体组分的结合（每0.1毫克蛋白质的百分比）为5.5±0.4%，Scatchard图呈直线；解离常数（Kd） = 5.6纳摩尔，最大结合容量（Ro） = 3.06皮摩尔/毫克；仅为大鼠的一半。在1例肝癌病例中，胰岛素与微粒体的结合正常，但EGF的结合非常低。
